FB2026_03 , released September 17, 2026
Allele: Dmel\TpihTPI.M82T
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General Information
Symbol
Dmel\TpihTPI.M82T
Species
D. melanogaster
Name
FlyBase ID
FBal0327051
Feature type
allele
Associated gene
Dmel\Tpi
Associated Insertion(s)
TI{TI}TpihTPI.M82T
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Roland et al., 2016)
Progenitor genotype
TpiattP
(Roland et al., 2016)
Associated Insertion(s)
TI{TI}TpihTPI.M82T
Cytology
Description

The endogenous Tpi coding region has been removed. A mutated Hsap\TPI1 coding sequence carrying the M82T amino acid replacement has been inserted in its place by phiC31-mediated integration into the attP site present in TpiattP.

(Roland et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      One copy of Hsap\TPI1M82T.Tpi (in which the coding sequence of endogenous Tpi is replaced by the human ortologue bearing the M82T amino-acid substitution) in a Tpi-null background (TpihTPI.M82T/Tpisgk-js10) leads to adult exhibiting a small decrease in lifespan, a small increase in bang sensitivity, shown by a small but significant increase in recovery time from mechanical stress, and an increase in heat sensitivity, shown by the significant decrease in the time until paralysis after a temperature shift to 38[o]C, as compared to controls.

      (Roland et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      TpihTPI.M82T
      Name Synonyms
      Secondary FlyBase IDs
        References (1)