Silencing (using Ctet\TeTxLC^TNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT045969 in adult males results in a significant reduction in the number of males that sing courtship song to females, with significant alterations in some parameters of pulse song, but not sine song, and occasional altered wing posture (drooping), compared to controls; there is no overall effect on the pattern of wing extension toward females and males with drooping wings still perform this behavior; copulation success is not significantly affected; take off and forced flight ability are significantly impaired, compared to controls.

Silencing (using Ctet\TeTxLC^TNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.VT045663 and Hsap\RELA^AD.VT045969 in adult males significantly alters some parameters of pulse song, but not sine song, compared to controls; there is no significant effect on the number of males that sing, wing extension toward females, copulation success, or take off and forced flight ability compared to controls.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 590 nm (yellow) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT045969 in adult males results in wing lifting.