Open Close
General Information
Symbol
Scer\GAL4DBD.R20G03
Species
S. cerevisiae
Name
FlyBase ID
FBal0331441
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The GMR20G03 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
larval Wave neuron

Comment: when combined with P{R12C11-p65.AD} (combination referred to as 'MB120B')

Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 660 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R20G03 and Hsap\RELAAD.R12C11 in larvae significantly induces rolling behaviors, compared to controls; when optogenetic activation is restricted to small clones of these cells (using hs-FLP to excise a stop codon from Cnoc\ChR1Cs.20xUAS.FRT.Venus to generate Cnoc\ChR1Cs.20xUAS.Venus), activating a single larval abdominal 4 Wave neuron significantly increases forwards locomotion, whereas activating a single larval abdominal 3 Wave neuron significantly increases backwards locomotion, and activation of both of these neurons induces wiggling and bending (rolling behaviors), compared to controls.

Inhibition (using shi1.UAS at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R20G03 and Hsap\RELAAD.R12C11 in larvae significantly reduces the probability of backwards motion in response to a head touch and significantly increases the latency in transit to forwards locomotion, compared to controls, with no effect on the backwards response to blue light.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.R20G03.T:Zzzz\Zip-
Scer\GAL4DBD.R20G03
Name Synonyms
Secondary FlyBase IDs
    References (5)