FB2021_02, released April 13, 2021

Allele: Scer\GAL4DBD.R58E05

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General Information
Symbol
Scer\GAL4DBD.R58E05
Species
S. cerevisiae
Name
FlyBase ID
FBal0332142
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{R58E05-GAL4.DBD}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Rubin Laboratory at Janelia Farm, 2017)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{R58E05-GAL4.DBD}
      Cytology
      Nature of the lesion
      Statement
      Reference

      The GMR58E05 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

      (Rubin Laboratory at Janelia Farm, 2017)
      Allele components
      Product class / Tool use(s)
      Encoded product / tool
      GAL4(DBD)::Zip-
      Associated Sequence Features
      GMR58E05
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval abdominal excitatory interneuron eIN-3

      Comment: when combined with P{R21F05-p65.AD} (combination referred to as 'SS01379')

      (Zwart et al., 2016)
      adult stage
      descending neuron of the gnathal ganglion DNg30

      Comment: when combined with P{R60B12-p65.AD} (combination referred to as 'SS02387')

      (Namiki et al., 2018)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Acute, high intensity optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R58E05 and Hsap\RELAAD.R21F05 in larvae induces contraction of transverse muscle LT2, but not longitudinal muscle LO1, compared to baseline; chronic, low-level stimulation causes transverse muscle LT2 to contract earlier than normal in the locomotor cycle, reducing the phase offset between LT2 and LO1 contractions, compared to baseline.

      (Zwart et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.R58E05.T:Zzzz\Zip-
      Scer\GAL4DBD.R58E05
      Name Synonyms
      Secondary FlyBase IDs
        References (4)