FlyBase Allele Report: Scer\GAL4[DBD.R79H02]

General Information

Symbol

Scer\GAL4^DBD.R79H02

Species

S. cerevisiae

Name

FlyBase ID

FBal0332438

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R79H02-GAL4.DBD}

Also Known As

R79H02-ZpGdbd, R79H02_ZpGdbd

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R79H02-GAL4.DBD}

Cytology

Description

The GMR79H02 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR79H02

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

medulla intrinsic neuron Mi4

Comment: when combined with P{R48A07-p65.AD}

(Strother et al., 2018, Strother et al., 2017)

giant fiber neuron

Comment: when combined with P{R14A01-p65.AD} (combination referred to as 'SS00727')

when combined with P{R25C08-p65.AD} (combination referred to as 'SS00728')

(Namiki et al., 2018, von Reyn et al., 2017)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal jump response | adult stage, with Hsap\KCNJ2^{IVS.10xUAS.EGFP}, Hsap\RELA^AD.R17A04

(von Reyn et al., 2017)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R14A01

(von Reyn et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 590 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R79H02 and Hsap\RELA^AD.R14A01 in adults significantly increases takeoff rates, compared to controls; takeoffs are shorter in duration than spontaneous takeoffs in controls.

Silencing (using Hsap\KCNJ2^{IVS.10xUAS.EGFP}) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R79H02 and Hsap\RELA^AD.R14A01 in adults mildly reduces overall takeoff rates, and significantly and substantially reduces the proportion of short takeoffs, compared to controls.

(von Reyn et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions