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General Information
Symbol
Scer\GAL4DBD.VT029310
Species
S. cerevisiae
Name
FlyBase ID
FBal0333985
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The VT029310 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
dorsal tp motor neuron

Comment: when combined with P{VT022025-p65.AD} (combination referred to as 'tp1-SG')

when combined with P{VT040232-p65.AD} (combination referred to as 'tp1,tp2-SG')

ventral tp motor neuron

Comment: when combined with P{VT040232-p65.AD} (combination referred to as 'tp1,tp2-SG')

Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Silencing (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT022025, or the split-GAL4 pair of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232, in adult males results in a significant reduction in the number of males that sing courtship song to females, with significant alterations in some parameters of pulse song, but not sine song, compared to controls; take off and forced flight ability are also significantly impaired; in the case of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT022025 there is no effect on wing extension toward females; in the case of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232, but not Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT022025, most individuals have altered wing posture (drooping) and a significant reduction in copulation success, compared to controls.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (yellow) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232 in adult males results in wing lifting.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.VT029310.T:Zzzz\Zip-
Scer\GAL4DBD.VT029310
Name Synonyms
Secondary FlyBase IDs
    References (3)