FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4DBD.VT029310
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General Information
Symbol
Scer\GAL4DBD.VT029310
Species
S. cerevisiae
Name
FlyBase ID
FBal0333985
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{VT029310-GAL4.DBD}
Also Known As
VT029310.ZpGAL4DBD
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Rubin Laboratory at Janelia Farm, 2017)
Progenitor genotype
Carried in construct
P{VT029310-GAL4.DBD}
Cytology
Description

The VT029310 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)
Allele components
Component
Use(s)
Regulatory region(s)
VT029310
Encoded product / tool
GAL4(DBD)::Zip-
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      dorsal tp motor neuron

      Comment: when combined with P{VT022025-p65.AD} (combination referred to as 'tp1-SG')

      when combined with P{VT040232-p65.AD} (combination referred to as 'tp1,tp2-SG')

      (Dickerson et al., 2019, O'Sullivan et al., 2018)
      ventral tp motor neuron

      Comment: when combined with P{VT040232-p65.AD} (combination referred to as 'tp1,tp2-SG')

      (O'Sullivan et al., 2018)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Silencing (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT022025, or the split-GAL4 pair of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232, in adult males results in a significant reduction in the number of males that sing courtship song to females, with significant alterations in some parameters of pulse song, but not sine song, compared to controls; take off and forced flight ability are also significantly impaired; in the case of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT022025 there is no effect on wing extension toward females; in the case of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232, but not Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT022025, most individuals have altered wing posture (drooping) and a significant reduction in copulation success, compared to controls.

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (yellow) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232 in adult males results in wing lifting.

      (O'Sullivan et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.VT029310.T:Zzzz\Zip-
      Scer\GAL4DBD.VT029310
      Name Synonyms
      Secondary FlyBase IDs
        References (5)