The VT047316 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
Comment: when combined with P{VT010054-p65.AD} (combination referred to as 'hg1-SG1')
Silencing (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT047316 and Hsap\RELAAD.VT010054 in adult males significantly alters some parameters of pulse song and sine song, compared to controls; there is no significant effect on the number of males that sing, wing extension toward females, copulation success, or take off and forced flight ability compared to controls.