FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ecol\lexASTaR.FRT.Stop.V5-2A-LexA-VP16
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General Information
Symbol
Ecol\lexASTaR.FRT.Stop.V5-2A-LexA-VP16
Species
E. coli
Name
FlyBase ID
FBal0336369
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
Carried in Construct
PBac{brp(FRT.Stop)V5-2A-LexA-VP16}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Chen et al., 2014)
Progenitor genotype
Carried in construct
PBac{brp(FRT.Stop)V5-2A-LexA-VP16}
Cytology
Description

The PBac{brp(FRT.Stop)V5-2A-LexA-VP16} construct contains brp regulatory and coding sequences (genomic fragment of ~50kb) in which the stop codon has been replaced by an FRT cassette containing transcriptional and translational stop sequences, followed by a Tag:V5 tag, the 2A peptide and a lexA::VP16 driver sequence. Sequences downstream of the stop cassette are not translated, and thus the brp protein produced is not tagged and no lexA::VP16 protein is produced. In the presence of FLP recombinase the FRT cassette is excised. The brp protein produced is now tagged with Tag:V5, and the presence of the 2A peptide allows the lexA::VP16 driver to be cotranslated as a separate protein, under the control of the brp regulatory sequences.

(Chen et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
brp
Encoded product / tool
lexA::VP16
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (5)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ecol\lexASTaR.FRT.Stop.V5-2A-LexA-VP16
      Name Synonyms
      Secondary FlyBase IDs
        References (3)