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FB2026_01
,
released March 12, 2026
The PBac{brp(FRT.Stop)V5-2A-LexA-VP16} construct contains brp regulatory and coding sequences (genomic fragment of ~50kb) in which the stop codon has been replaced by an FRT cassette containing transcriptional and translational stop sequences, followed by a Tag:V5 tag, the 2A peptide and a lexA::VP16 fusion. Sequences downstream of the stop cassette are not translated, and thus the brp protein produced is not tagged and no lexA::VP16 protein is produced. In the presence of FLP recombinase, the FRT cassette is excised. The brp protein produced is now tagged with Tag:V5, and the presence of the 2A peptide allows the lexA::VP16 fusion to be cotranslated as a separate protein, under the control of the brp regulatory sequences.
The PBac{brp(FRT.Stop)V5-2A-LexA-VP16} construct contains brp regulatory and coding sequences (genomic fragment of ~50kb) in which the stop codon has been replaced by an FRT cassette containing transcriptional and translational stop sequences, followed by a Tag:V5 tag, the 2A peptide and a lexA::VP16 driver sequence. Sequences downstream of the stop cassette are not translated, and thus the brp protein produced is not tagged and no lexA::VP16 protein is produced. In the presence of FLP recombinase the FRT cassette is excised. The brp protein produced is now tagged with Tag:V5, and the presence of the 2A peptide allows the lexA::VP16 driver to be cotranslated as a separate protein, under the control of the brp regulatory sequences.
The PBac{brp(FRT.Stop)V5-2A-LexA-VP16} construct contains brp regulatory and coding sequences (genomic fragment of ~50kb) in which the stop codon has been replaced by an FRT cassette containing transcriptional and translational stop sequences, followed by a T:SV5\V5 tag, the 2A peptide and a lexA::VP16 fusion. Sequences downstream of the stop cassette are not translated, and thus the brp protein produced is not tagged and no lexA::VP16 protein is produced. In the presence of FLPase, the FRT cassette is excised. The brp protein produced is now tagged with T:SV5\V5, and the presence of the 2A peptide allows the lexA::VP16 fusion to be cotranslated as a separate protein, under the control of the brp regulatory sequences.