FB2026_02 , released June 18, 2026
Allele: Dmel\GαqV303D
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General Information
Symbol
Dmel\GαqV303D
Species
D. melanogaster
Name
FlyBase ID
FBal0337856
Feature type
allele
Associated gene
Dmel\Gαq
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Amino acid replacement: V303D.

    (Cao et al., 2018)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    2R:12,618,240..12,618,240 [+]
    Nucleotide change:

    T12618240A

    Amino acid change:

    V303D | Galphaq-PD

    Reported amino acid change:

    V303D

    Comment:

    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The mutation is specific to the Galphaq-PD isoform.

    (Cao et al., 2018)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        GαqV303D homozygous as well as GαqV303D/Df(2R)Gαq1.3 or GαqV303D/Df(2R)Exel7121 transheterozygous adults show no electroretinogram (ERG) response to light stimulation. The GαqV303D mutants also display only a minimal response to a very bright illumination and their retinal tissue show rapid light-dependent degeneration (after six days in light/dark cycles the rhabdomeres in the mutant retina are largely missing or severely damaged), the ommatidial structure is intact in 1-day-old flies or flies kept in darkness.

        (Cao et al., 2018)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        NOT suppressed by
        Statement
        Reference

        GαqV303D has retina | adult stage phenotype, non-suppressible by CalxA/CalxA

        (Cao et al., 2018)

        GαqV303D has rhabdomere | adult stage phenotype, non-suppressible by CalxA/CalxA

        (Cao et al., 2018)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        The rapid light-dependent retinal degeneration characteristic observed in GαqV303D adult mutants is not rescued by combination with CalxA.

        (Cao et al., 2018)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        GαqV303D is rescued by Scer\GAL4GMR.long/GαqUAS.cCa

        (Cao et al., 2018)

        GαqV303D is rescued by Scer\GAL4GMR.long/GαqV303I.UAS

        (Cao et al., 2018)
        Not rescued by

        GαqV303D is not rescued by GαqV303D.UAS/Scer\GAL4GMR.long

        (Cao et al., 2018)
        Comments

        The GαqV303D allele cannot be complemented by either Df(2R)Gαq1.3 or Df(2R)Exel7121 as they cannot rescue the flat electroretinogram (ERG) response to light stimulation observed in GαqV303D mutants, GαqV303D/Df(2R)BSC485 transheterozygotes show normal ERG response.

        Scer\GAL4GMR.long-driven expression of Gαq.Scer\UAS (but not GαqV303D.Scer\UAS) can rescue the loss of response to light stimulation (flat electroretinogram, ERG, response) along with the rapid light-dependent retinal degeneration characteristic for GαqV303D adult flies. Expression of GαqV303I.Scer\UAS also rescues the lack of light response in ERG measurements.

        (Cao et al., 2018)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (2)
        Reported As
        Symbol Synonym
        GαqV303D
        (Cao et al., 2018)
        GαqV303D
        Name Synonyms
        Secondary FlyBase IDs
          References (1)