FlyBase Allele Report: Dmel\RpS12[+t2]

General Information

Symbol

Dmel\RpS12^+t2

Species

D. melanogaster

Name

FlyBase ID

FBal0337981

Feature type

allele

Associated gene

Dmel\RpS12

Associated Insertion(s)

Carried in Construct

PBac{RpS12.2kb}

Key Links

Transgenic product class

genomic_DNA

(Kale et al., 2018)

wild_type

(Kale et al., 2018)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Kale et al., 2018)

wild_type

(Kale et al., 2018)

Mutagen

in vitro construct

(Kale et al., 2018)

Progenitor genotype

Carried in construct

PBac{RpS12.2kb}

Cytology

Description

An approximately 2kb genomic fragment containing the wild type RpS12 locus, which includes snoRNA:Me28S-A774a and snoRNA:Me28S-A774b in its introns.

(Kale et al., 2018)

Allele components

Component

Use(s)

Regulatory region(s)

RpS12

Encoded product / tool

RpS12

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Larval wing disc clones bearing one or two copies of RpS12^+t2 do not compete with neighboring wild-type control clones, as shown by their comparable sizes.

(Kale et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

RpS12^+t2 is an enhancer of increased cell death | dominant | somatic clone phenotype of M(2)56F^H

(Kale et al., 2018)

Other

Statement

Reference

Df(1)su(s)R194/+, RpS12^+t2, RpS12^s2783 has abnormal developmental rate phenotype

(Kale et al., 2018)

Df(1)su(s)R194/+, RpS12^+t2, RpS12^s2783 has Minute phenotype

(Kale et al., 2018)

Df(2R)BSC865/+, RpS12^+t2 has decreased cell number | somatic clone | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^+t2 has decreased cell number | larval stage phenotype

(Kale et al., 2018)

Phenotype Manifest In

Enhancer of

Statement

Reference

RpS12^+t2 is an enhancer of wing disc | somatic clone phenotype of M(2)56F^H

(Kale et al., 2018)

Other

Statement

Reference

Df(2R)BSC865/+, RpS12^+t2 has wing disc | somatic clone | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^+t2 has wing disc | larval stage phenotype

(Kale et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Df(1)su(s)R194-heterozygous clones in the larval eye disc are outcompeted by neighboring clones that also harbor RpL36^+t4); this phenotype is not suppressed by either two copies of RpS12^+t2 alone or in combination with RpS12^s2783 homozygosity.

The following genotypes exhibit a minute-like developmental delay phenotype as compared to controls: Df(1)su(s)R194/+, RpS12^s2783/RpS12^s2783, RpS12^+t2.

In Df(2R)BSC865 or M(2)56F^H single heterozygous larval wing discs, clones bearing two copies of RpS12^+t2 are severely outcompeted by otherwise wild-type neighboring clones, as shown by their decreased clone size. The increased cell death observed in M(2)56F^H-heterozygous larval wing discs is enhanced within clones bearing two copies of RpS12^+t2, but only near the clone boundary.

(Kale et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

RpS12^+t2 rescues RpS12^s2783

(Kale et al., 2018)

Comments

Two copies of RpS12^+t2 rescue the cell lethality RpS12^s2783-homozygous clones in larval wing discs.

(Kale et al., 2018)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

RpS12^+t2

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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