FlyBase Allele Report: Dmel\M(2)56F[H]

General Information

Symbol

Dmel\M(2)56F^H

Species

D. melanogaster

Name

FlyBase ID

FBal0338100

Feature type

allele

Associated gene

M(2)56F

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | dominant

(Kale et al., 2018)

cell lethal | somatic clone

(Kale et al., 2015)

increased cell death | dominant | somatic clone

(Kale et al., 2018)

increased cell death | somatic clone

(Kale et al., 2015)

Minute | dominant

(Kale et al., 2018)

Phenotype Manifest In

macrochaeta

(Kale et al., 2018)

wing disc | somatic clone

(Kale et al., 2018)

Detailed Description

Statement

Reference

M(2)56F^H heterozygotes present a Minute phenotype, which includes a developmental delay and minute bristle phenotype, as compared to controls. M(2)56F^H heterozygous larval wing discs present increased cell death, as compared to controls.

(Kale et al., 2018)

Apoptosis occurs in at the interface of wild type and M(2)56F^H/+ cells in M(2)56F^H/+ wing discs in which wild type clones have been induced.

(Kale et al., 2015)

When +/+ clones are induced in a M(2)56F^H/+ background and cell death is analysed, apoptotic cells are largely restricted to boundaries between wild-type and M(2)56F^H/+ cell populations and the apoptotic cells are almost always M(2)56F^H/+. When +/+ clones are induced in a M(2)56F^H/+ background in the wing disc, dying cells are seen throughout the apical-basal axis of the epithelium, but are more often basal. Apoptotic material is sometimes present within neighbouring, wild-type epithelial cells, indicating that some of the apoptotic cells are cleared by engulfment into neighbouring cells.

(Li and Baker, 2007)

M(2)56i^unspecified does not show a dominant rough eye phenotype.

(Rodrigues et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

M(2)56F^H has increased cell death | dominant | somatic clone phenotype, enhanceable by RpS12^+t2

(Kale et al., 2018)

NOT Enhanced by

Statement

Reference

M(2)56F^H has increased cell death | dominant | somatic clone phenotype, non-enhanceable by RpS12^G97D.2kb

(Kale et al., 2018)

NOT suppressed by

Statement

Reference

M(2)56F^H has Minute | dominant phenotype, non-suppressible by RpS12^G97D/RpS12[+]

(Kale et al., 2018)

M(2)56F^H has Minute | dominant phenotype, non-suppressible by RpS12^s2783/RpS12^G97D

(Kale et al., 2018)

M(2)56F^H has abnormal developmental rate | dominant phenotype, non-suppressible by RpS12^G97D/RpS12[+]

(Kale et al., 2018)

M(2)56F^H has abnormal developmental rate | dominant phenotype, non-suppressible by RpS12^s2783/RpS12^G97D

(Kale et al., 2018)

M(2)56F^H has increased cell death | somatic clone phenotype, non-suppressible by p53^5A-1-4/p53^11-1B-1

(Kale et al., 2015)

M(2)56F^H has cell lethal | somatic clone phenotype, non-suppressible by Dredd^B118/Dredd^B118

(Kale et al., 2015)

M(2)56F^H has increased cell death | somatic clone phenotype, non-suppressible by Dredd^B118/Dredd^B118

(Kale et al., 2015)

Other

Statement

Reference

M(2)56F^H/M(2)56F[+], RpS12^s2783 has increased cell number | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^G97D has decreased cell number | somatic clone | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^+t2 has decreased cell number | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^G97D.2kb has decreased cell number | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^UAS.Tag:FLAG, Scer\GAL4^Act5C.PP has decreased cell number | larval stage phenotype

(Kale et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

M(2)56F^H has wing disc | somatic clone phenotype, enhanceable by RpS12^+t2

(Kale et al., 2018)

NOT Enhanced by

Statement

Reference

M(2)56F^H has wing disc | somatic clone phenotype, non-enhanceable by RpS12^G97D.2kb

(Kale et al., 2018)

NOT suppressed by

Statement

Reference

M(2)56F^H has macrochaeta phenotype, non-suppressible by RpS12^G97D/RpS12[+]

(Kale et al., 2018)

M(2)56F^H has macrochaeta phenotype, non-suppressible by RpS12^s2783/RpS12^G97D

(Kale et al., 2018)

NOT Suppressor of

Statement

Reference

M(2)56F^H/M(2)56F[+] is a non-suppressor of wing disc | somatic clone | larval stage phenotype of RpS12^s2783

(Kale et al., 2018)

Other

Statement

Reference

M(2)56F^H/M(2)56F[+], RpS12^G97D has wing disc | somatic clone | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^+t2 has wing disc | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^G97D.2kb has wing disc | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^UAS.Tag:FLAG, Scer\GAL4^Act5C.PP has wing disc | somatic clone | larval stage phenotype

(Kale et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The minute phenotype observed in M(2)56F^H heterozygotes (i.e. developmental delay and minute bristles) is not suppressed by RpS12^G97D heterozygosity or RpS12^G97D/RpS12^s2783 transheterozygosity.

In M(2)56F^H-heterozygous larval wing discs, otherwise wild-type clones are outcompeted by neighboring RpS12^G97D-homozygous clones, severely outcompete neighboring clones either bearing two copies of RpS12^+t2 or expressing RpS12^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PP, and do not compete with neighboring clones either bearing two copies of RpS12^G97D.2kb or expressing RpS12^{G97D.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act5C.PP. The increased cell death observed in M(2)56F^H-heterozygous larval wing discs is enhanced within clones bearing two copies of RpS12^+t2, but not in clones bearing two copies of RpS12^G97D.2kb; this enhancement is only observed near the clone boundary.

M(2)56F^H, RpS12^s2783 double heterozygous larval wing disc cells outcompete M(2)56F^H single heterozygous cells, as the single heterozygous clones are rarely recovered within the double heterozygous background.

(Kale et al., 2018)

The apoptosis that occurs at the interface of wild type and M(2)56F^H/+ cells in M(2)56F^H/+ wing discs in which wild type clones have been induced is not significantly lowered in discs that are also mutant for either p53^11-1B-1/p53^5A-1-4 or homozygous for Dredd^B118.

(Kale et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Obtained from laboratory of Y. Hiromi.

(Baker, 2018.2.7)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

M(2)56F

(Kale et al., 2018)

M(2)56F^H

(Baker, 2018.2.7)

M(2)56i^unspecified

M(2R)56f

(Kale et al., 2018)

Name Synonyms

Hiromi

Secondary FlyBase IDs

FBal0337912
FBal0193569

References (7)

Report Sections

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