FlyBase Allele Report: Dmel\RpS12[s2783]

General Information

Symbol

Dmel\RpS12^s2783

Species

D. melanogaster

Name

FlyBase ID

FBal0028400

Feature type

allele

Associated gene

Dmel\RpS12

Associated Insertion(s)

P{lacW}RpS12^s2783

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}RpS12^s2783

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:13,023,409..13,023,409 [+]

(Walsh et al., 2025, Spradling et al., 1999, Netter et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing (with RpS12^G97D)

(Ji et al., 2019)

wing disc | larval stage | somatic clone

(Kale et al., 2018)

Detailed Description

Statement

Reference

RpS12^s2783 homozygotes and RpS12^s2783/RpS12^c06284 and RpS12^s2783/RpS12^EP3025 transheterozygotes are lethal. RpS12^G97D/RpS12^s2783 transheterozygotes are viable, do not shown any developmental delay (assessed by the time until eclosion), and lack any obvious morphological defects, including any minute bristle-like phenotype, as compared to controls.

RpS12^s2783-homozygous clones in larval wing discs are not recovered, suggesting that RpS12^s2783 is homozygous cell lethal. RpS12^s2783-heterozygous clones in larval wing discs are not outcompeted by neighboring wild-type clones.

(Kale et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

RpS12^s2783 has cell lethal | somatic clone | larval stage phenotype, non-suppressible by Df(2R)BSC865/+

(Kale et al., 2018)

Suppressor of

Statement

Reference

RpS12^s2783/RpS12[+] is a suppressor | partially of decreased size | adult stage phenotype of Hsap\RPS12^UAS.cKa, Scer\GAL4^GMR.PU

(Katanaev et al., 2020)

RpS12^s2783/RpS12[+] is a suppressor | partially of visible | adult stage phenotype of Hsap\RPS12^UAS.cKa, Scer\GAL4^GMR.PU

(Katanaev et al., 2020)

RpS12^s2783 is a suppressor | partially of abnormal developmental rate phenotype of RpS18^unspecified

(Ji et al., 2019)

RpS12^s2783/RpS12[+] is a suppressor of visible phenotype of CycE^JP

(Lin et al., 2011)

NOT Suppressor of

Statement

Reference

RpS12^s2783/RpS12^G97D is a non-suppressor of decreased cell number | somatic clone | larval stage phenotype of Myc^αTub84B.PBb

(Kale et al., 2018)

RpS12^s2783/RpS12^G97D is a non-suppressor of Minute | dominant phenotype of M(2)56F^H

(Kale et al., 2018)

RpS12^s2783/RpS12^G97D is a non-suppressor of abnormal developmental rate | dominant phenotype of M(2)56F^H

(Kale et al., 2018)

Other

Statement

Reference

Df(1)su(s)R194/+, RpS12^s2783 has abnormal developmental rate | dominant phenotype

(Kale et al., 2018)

Df(1)su(s)R194/+, RpS12^s2783 has abnormal developmental rate phenotype

(Kale et al., 2018)

Df(1)su(s)R194/+, RpS12^s2783 has Minute phenotype

(Kale et al., 2018)

Df(2R)BSC865/+, RpS12^s2783 has increased cell number | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^s2783 has increased cell number | larval stage phenotype

(Kale et al., 2018)

Df(1)su(s)R194/+, RpS12^+t2, RpS12^s2783 has abnormal developmental rate phenotype

(Kale et al., 2018)

Df(1)su(s)R194/+, RpS12^+t2, RpS12^s2783 has Minute phenotype

(Kale et al., 2018)

Df(1)su(s)R194/+, RpS12^G97D.2kb, RpS12^s2783 has abnormal developmental rate phenotype

(Kale et al., 2018)

Df(1)su(s)R194/+, RpS12^G97D.2kb, RpS12^s2783 has Minute phenotype

(Kale et al., 2018)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

RpS12^s2783 has wing disc | somatic clone | larval stage phenotype, non-suppressible by Df(2R)BSC865/+

(Kale et al., 2018)

RpS12^s2783 has wing disc | somatic clone | larval stage phenotype, non-suppressible by M(2)56F^H/M(2)56F[+]

(Kale et al., 2018)

Suppressor of

Statement

Reference

RpS12^s2783/RpS12[+] is a suppressor | partially of eye phenotype of Hsap\RPS12^UAS.cKa, Scer\GAL4^GMR.PU

(Katanaev et al., 2020)

RpS12^s2783/RpS12[+] is a suppressor of eye phenotype of CycE^JP

(Lin et al., 2011)

NOT Suppressor of

Statement

Reference

RpS12^s2783/RpS12^G97D is a non-suppressor of wing disc | somatic clone | larval stage phenotype of Myc^αTub84B.PBb

(Kale et al., 2018)

RpS12^s2783/RpS12^G97D is a non-suppressor of macrochaeta phenotype of M(2)56F^H

(Kale et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Df(1)su(s)R194-heterozygous clones in the larval eye disc are outcompeted by neighboring clones that also harbor RpL36^+t4); this phenotype is not suppressed by homozygosity for RpS12^s2783 alone or in combination with RpS12^+t2.

RpS12^G97D/RpS12^s2783 transheterozygosity do not suppress the minute phenotype observed in M(2)56F^H heterozygotes (i.e. developmental delay and minute bristles). The following genotypes also result in a minute-like developmental delay phenotype as compared to controls: Df(1)su(s)R194/+, RpS12^s2783/+; Df(1)su(s)R194/+, RpS12^s2783/RpS12^s2783; Df(1)su(s)R194/+, RpS12^s2783/RpS12^s2783, RpS12^+t2; or Df(1)su(s)R194/+, RpS12^s2783/RpS12^s2783, RpS12^G97D.2kb.

The cell lethality of RpS12^s2783 is not suppressed by Df(2R)BSC865, as RpS12^s2783-homozygous clones in larval wing discs are still not recovered in a Df(2R)BSC865 heterozygous background.

Df(2R)BSC865, RpS12^s2783 and M(2)56F^H, RpS12^s2783 double heterozygous larval wing disc cells outcompete Df(2R)BSC865 and M(2)56F^H single heterozygous cells, respectively, as single heterozygous clones are rarely recovered within the respective double heterozygous background.

Myc^αTub84B.PBb homozygous clones in larval wing discs are outcompeted by neighboring wild-type clones; this phenotype is not suppressed by a RpS12^G97D/RpS12^s2783 transheterozygous background.

(Kale et al., 2018)

The eye phenotype seen in homozygous CycE^JP adults is moderately suppressed if they are also heterozygous for RpS12^s2783.

(Lin et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

RpS12^s2783/RpS12^EP3025 is rescued by RpS12^Δ2-3.6kb

(Kale et al., 2018)

RpS12^s2783 is rescued by RpS12^+t2

(Kale et al., 2018)