FlyBase Allele Report: Dmel\RpS12[G97D]

General Information

Symbol

Dmel\RpS12^G97D

Species

D. melanogaster

Name

FlyBase ID

FBal0193403

Feature type

allele

Associated gene

Dmel\RpS12

Associated Insertion(s)

Carried in Construct

Also Known As

su(comp)3L-1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tyler et al., 2007)

Progenitor genotype

Cytology

Description

Amino acid replacement: G97D.

(Kale et al., 2018)

Nucleotide substitution coordinates are for Release 6.

(Kale et al., 2018)

Nucleotide substitution: G13024507A.

(Kale et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:13,024,507..13,024,507 [+]

Nucleotide change:

G13024507A

Amino acid change:

G97D | RpS12-PB

Reported amino acid change:

G97D

(Kale et al., 2018)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Ji et al., 2019)

abnormal developmental rate (with RpS12^EP3025)

(Ji et al., 2019)

decreased size | adult stage (with RpS12^s2783)

(Ji et al., 2019)

decreased size | adult stage | female

(Ji et al., 2019)

short lived

(Ji et al., 2019)

viable

(Kale et al., 2018)

viable (with RpS12^c06284)

(Kale et al., 2018)

viable (with RpS12^EP3025)

(Kale et al., 2018)

viable (with RpS12^s2783)

(Kale et al., 2018)

Phenotype Manifest In

wing (with RpS12^s2783)

(Ji et al., 2019)

wing | female

(Ji et al., 2019)

Detailed Description

Statement

Reference

RpS12^G97D homozygotes and RpS12^G97D/RpS12^s2783, RpS12^G97D/RpS12^c06284 and RpS12^G97D/RpS12^EP3025 transheterozygotes are viable and lack any obvious morphological defects. RpS12^G97D heterozygotes or RpS12^G97D/RpS12^s2783 transheterozygotes also do not show any developmental delay (assessed by the time until eclosion) or any minute bristle-like phenotype, as compared to controls.

RpS12^s2783-homozygous clones in larval wing discs do not compete with neighboring wild-type control clones, as shown by their comparable sizes.

(Kale et al., 2018)

su(comp)3L-1^3L-1 has no effect on the apoptotic response seen in the eye discs containing dicentric chromosomes (generated using Scer\FLP1^hs.PP-induced recombination in chromosomes containing inverted Scer\FRT sites). This effect is seen at 12-14 hours after heat shock of DcYy⁺.

(Titen and Golic, 2008)

Homozygous clones in the eye do not outgrow their twin spots.

(Tyler et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

RpS12^G97D/RpS12^G97D is an enhancer of abnormal developmental rate phenotype of RpS3^unspecified, Xrp1^M2-73

(Ji et al., 2019)

NOT Enhancer of

Statement

Reference

RpS12^G97D is a non-enhancer of abnormal developmental rate phenotype of RpS17⁴

(Ji et al., 2019)

RpS12^G97D is a non-enhancer of abnormal developmental rate phenotype of RpS17⁴, Xrp1^M2-73

(Ji et al., 2019)

Suppressor of

Statement

Reference

RpS12^G97D is a suppressor of abnormal developmental rate phenotype of RpS3^unspecified

(Ji et al., 2019)

Xrp1^M2-73, RpS12^G97D/RpS12^G97D is a suppressor of abnormal developmental rate phenotype of RpS3^unspecified

(Ji et al., 2019)

NOT Suppressor of

Statement

Reference

RpS12^G97D is a non-suppressor of abnormal developmental rate phenotype of RpS17⁴

(Ji et al., 2019)

RpS12^G97D is a non-suppressor of abnormal developmental rate phenotype of RpS17⁴, Xrp1^M2-73

(Ji et al., 2019)

RpS12^s2783/RpS12^G97D is a non-suppressor of decreased cell number | somatic clone | larval stage phenotype of Myc^αTub84B.PBb

(Kale et al., 2018)

RpS12^G97D/RpS12[+] is a non-suppressor of Minute | dominant phenotype of M(2)56F^H

(Kale et al., 2018)

RpS12^s2783/RpS12^G97D is a non-suppressor of Minute | dominant phenotype of M(2)56F^H

(Kale et al., 2018)

RpS12^G97D/RpS12[+] is a non-suppressor of abnormal developmental rate | dominant phenotype of M(2)56F^H

(Kale et al., 2018)

RpS12^s2783/RpS12^G97D is a non-suppressor of abnormal developmental rate | dominant phenotype of M(2)56F^H

(Kale et al., 2018)

Other

Statement

Reference

Df(1)su(s)R194/+, RpS12^G97D has abnormal developmental rate | dominant phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^G97D has decreased cell number | somatic clone | larval stage phenotype

(Kale et al., 2018)

Df(1)su(s)R194/+, RpS12^G97D has Minute phenotype

(Kale et al., 2018)

Df(2R)BSC865/+, RpS12^G97D has decreased cell number | somatic clone | larval stage phenotype

(Kale et al., 2018)

Df(2L)M24F11/+, RpS12^G97D has decreased cell number | somatic clone | larval stage phenotype

(Kale et al., 2018)

Df(2R)M60E/+, RpS12^G97D has decreased cell number | somatic clone | larval stage phenotype

(Kale et al., 2018)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

RpS12^s2783/RpS12^G97D is a non-suppressor of wing disc | somatic clone | larval stage phenotype of Myc^αTub84B.PBb

(Kale et al., 2018)

RpS12^G97D/RpS12[+] is a non-suppressor of macrochaeta phenotype of M(2)56F^H

(Kale et al., 2018)

RpS12^s2783/RpS12^G97D is a non-suppressor of macrochaeta phenotype of M(2)56F^H

(Kale et al., 2018)

Other

Statement

Reference

RpS12^G97D, RpS3^unspecified has wing disc | somatic clone | larval stage phenotype

(Ji et al., 2019)

Df(2R)BSC865/+, RpS12^G97D has wing disc | somatic clone | larval stage phenotype

(Kale et al., 2018)

Df(2L)M24F11/+, RpS12^G97D has wing disc | somatic clone | larval stage phenotype

(Kale et al., 2018)

Df(2R)M60E/+, RpS12^G97D has wing disc | somatic clone | larval stage phenotype

(Kale et al., 2018)

M(2)56F^H/M(2)56F[+], RpS12^G97D has wing disc | somatic clone | larval stage phenotype

(Kale et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Df(1)su(s)R194-heterozygous clones in the larval eye disc are outcompeted by neighboring clones that also harbor RpL36^+t4); this phenotype is suppressed by homozygosity for RpS12^G97D alone or in combination with RpS12^G97D.2kb.

In Df(2R)BSC865, Df(2L)M24F11, Df(2R)M60E or M(2)56F^H single heterozygous larval wing discs, RpS12^G97D-homozygous clones outcompete otherwise wild-type neighboring clones, as shown by their significant relative increase in clone size.

RpS12^G97D heterozygosity or RpS12^G97D/RpS12^s2783 transheterozygosity do not suppress the minute phenotype observed in M(2)56F^H heterozygotes (i.e. developmental delay and minute bristles). Df(1)su(s)R194, RpS12^G97D double transheterozygotes also present a minute-like developmental delay phenotype compared to controls.

Myc^αTub84B.PBb homozygous clones in larval wing discs are outcompeted by neighboring wild-type clones; this phenotype is not suppressed by a RpS12^G97D/RpS12^s2783 transheterozygous background.

(Kale et al., 2018)

su(comp)3L-1^3L-1 is a recessive, cell-autonomous mutation that allows recovery of Df(1)su(s)R194/+ clones in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36^+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Cell death is much reduced in Df(1)su(s)R194/+ ; su(comp)3L-1^3L-1 clones compared to Df(1)su(s)R194/+ clones.

(Tyler et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

RpS12^G97D is rescued by RpS12^+t8

(Ji et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: A mutation that allows survival of cells being competed due to haplo-insufficiency for RpL36.

(Tyler et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

RpS12^G97D

rpS12^97D

(Ji et al., 2019)

rpS12^D97

(Ji et al., 2019)

rpS12^G97D

(Ji et al., 2021, Kale et al., 2018, Lee et al., 2018)

su(comp)3L-1

(Kale et al., 2018, Tyler et al., 2007)

su(comp)3L-1^3L-1

Name Synonyms

Secondary FlyBase IDs

References (6)

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