FlyBase Allele Report: Dmel\Gyc76C[RNAi.UAS.cUa]

General Information

Symbol

Dmel\Gyc76C^RNAi.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0338852

Feature type

allele

Associated gene

Dmel\Gyc76C

Associated Insertion(s)

Carried in Construct

P{UAS-Gyc76C.RNAi.U}

Key Links

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Gyc76C.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of a Gyc76C RNAi construct.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Gyc76C

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

extracellular matrix | pupal stage, with Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

presumptive wing vein | pupal stage, with Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

wing disc | pupal stage, with Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

Detailed Description

Statement

Reference

Expression of Gyc76C^VDRC.cUa under the control of Scer\GAL4^hh.PU leads to defects in venation pattern and extracellular matrix organization in the pupal wings from 24 hr after pupariation onwards.

(Schleede and Blair, 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU has visible | adult stage phenotype, suppressible by trol^GS7407, Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

Suppressor of

Statement

Reference

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^hh.PU, trol^GS7407

(Schleede and Blair, 2015)

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^dpp.3.7XK is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^dpp.3.7XK, dpp^UAS.cUa.GFP

(Schleede and Blair, 2015)

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^en.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^en.PU, sog^UAS.Tag:HA

(Schleede and Blair, 2015)

Other

Statement

Reference

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU, trol^GS7407 has visible | adult stage phenotype

(Schleede and Blair, 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU has presumptive wing vein | pupal stage phenotype, suppressible by Timp^UAS.cPa, Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU has wing disc | pupal stage phenotype, suppressible by Mmp2^RNAi.UAS, Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU has extracellular matrix | pupal stage phenotype, suppressible by Mmp2^RNAi.UAS, Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU has presumptive wing vein | pupal stage phenotype, suppressible by Mmp2^RNAi.UAS, Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU has posterior crossvein phenotype, suppressible by trol^GS7407, Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

NOT suppressed by

Statement

Reference

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU has wing disc | pupal stage phenotype, non-suppressible by Mmp1^RNAi.UAS, Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

Suppressor of

Statement

Reference

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^dpp.3.7XK is a suppressor | partially of wing vein L5 phenotype of Scer\GAL4^dpp.3.7XK, dpp^UAS.cUa.GFP

(Schleede and Blair, 2015)

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^en.PU is a suppressor of posterior crossvein phenotype of Scer\GAL4^en.PU, sog^UAS.Tag:HA

(Schleede and Blair, 2015)

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU is a suppressor of posterior crossvein phenotype of Scer\GAL4^hh.PU, trol^GS7407

(Schleede and Blair, 2015)

Other

Statement

Reference

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU, trol^GS7407 has wing blade phenotype

(Schleede and Blair, 2015)

Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU, Timp^UAS.cPa has extracellular matrix | pupal stage phenotype

(Schleede and Blair, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The disrupted formation of the presumptive posterior crossvein (assessed by p-Mad staining) in the pupal wing observed in animals expressing Gyc76C^VDRC.cUa under the control of Scer\GAL4^hh.PU is rescued by co-expression of Timp^Scer\UAS.cPa but it also leads to aberrant accumulations of the extracellular matrix. Co-expression of Mmp2^{dsRNA.Scer\UAS} but not Mmp1^{dsRNA.Scer\UAS} also rescues the pupal wing disc phenotypes, although the Mmp2^{dsRNA.Scer\UAS},Gyc76C^VDRC.cUa double knock-down larvae and pupae are occasionally unhealthy-looking and those individuals also have pupal wings of poor morphology.

The widening of the wing vein L5 characteristic for adult flies expressing dpp^{Scer\UAS.cUa.T:Avic\GFP} under the control of Scer\GAL4^dpp.3.7XK is partially suppressed by co-expression of Gyc76C^VDRC.cUa.

The loss of posterior crossvein observed in adult flies expressing sog^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en.PU is rescued by co-expression of Gyc76C^VDRC.cUa.

The widening of the posterior crossvein observed in the wings of adult flies expressing trol^GS7407 under the control of Scer\GAL4^hh.PU is suppressed by co-expression of Gyc76C^VDRC.cUa (which on its own disrupts posterior crossvein development) and results in fairly normal crossvein but the wings display significant blistering.

(Schleede and Blair, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Gyc76C^RNAi.UAS.cUa

Gyc76C^VDRC.cUa

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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