FlyBase Allele Report: Dmel\Mmp1[RNAi.UAS]

General Information

Symbol

Dmel\Mmp1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0212915

Feature type

allele

Associated gene

Dmel\Mmp1

Associated Insertion(s)

Carried in Construct

P{UAS-Mmp1.RNAi}

Also Known As

UAS-mmp1^RNAi, UAS-Mmp1-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Uhlirova and Bohmann, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Uhlirova and Bohmann, 2006)

Mutagen

in vitro construct

(Uhlirova and Bohmann, 2006)

Progenitor genotype

Carried in construct

P{UAS-Mmp1.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of Mmp1. The repeat is separated by a w intron.

(Uhlirova and Bohmann, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mmp1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates carcinoma

modeled by Ras85D^V12.UAS, scrib¹

(Uhlirova and Bohmann, 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response | adult stage, with Scer\GAL4^repo

(Hsu et al., 2021)

Phenotype Manifest In

axon | adult stage, with Scer\GAL4^repo

(Hsu et al., 2021)

embryonic/larval tracheal dorsal trunk, with Scer\GAL4^btl.PS

(Uhlirova and Bohmann, 2006)

wing nerve, with Scer\GAL4^repo

(Hsu et al., 2021)

Detailed Description

Statement

Reference

Knockdown of Mmp1^{dsRNA.Scer\UAS} driven by Scer\GAL4^Act.PU causes a reduction in egg laying compared to wild type.

(Deady et al., 2015)

Expression of Mmp1^{dsRNA.Scer\UAS} under the simultaneous control of both Scer\GAL4^bab1-Agal4-5 and Scer\GAL4^ptc-559.1 (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to the adult stage) has no effect on the average number of cells in the interfollicle stalks.

(Wang and Page-McCaw, 2014)

Expression of Mmp1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^btl.PS does not perturb wing disc:tracheal association and dorsal air sac primordium growth.

(Guha et al., 2009)

Expression of Mmp1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^btl.PS results in constrictions and breaks in the dorsal trunk.

(Uhlirova and Bohmann, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/Mmp1^RNAi.UAS is a suppressor | partially of neoplasia phenotype of Sox21a⁶

(Zhai et al., 2015)

NOT Suppressor of

Statement

Reference

Mmp1^RNAi.UAS, Scer\GAL4^Act5C.PI is a non-suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, scrib¹

(Uhlirova and Bohmann, 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/Mmp1^RNAi.UAS is a suppressor | partially of adult anterior midgut epithelium phenotype of Sox21a⁶

(Zhai et al., 2015)

Mmp1^RNAi.UAS, Scer\GAL4^Act5C.PI is a suppressor | partially of larval ventral nerve cord | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, scrib¹

(Uhlirova and Bohmann, 2006)

Mmp1^RNAi.UAS, Scer\GAL4^Act5C.PI is a suppressor | partially of larval brain | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, scrib¹

(Uhlirova and Bohmann, 2006)

Scer\GAL4^Act5C.PI, Mmp1^RNAi.UAS/Mmp2^RNAi.UAS is a suppressor of larval brain | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, scrib¹

(Uhlirova and Bohmann, 2006)

Scer\GAL4^Act5C.PI, Mmp1^RNAi.UAS/Mmp2^RNAi.UAS is a suppressor of larval ventral nerve cord | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, scrib¹

(Uhlirova and Bohmann, 2006)

NOT Suppressor of

Statement

Reference

Mmp1^RNAi.UAS, Scer\GAL4^hh.PU is a non-suppressor of wing disc | pupal stage phenotype of Gyc76C^RNAi.UAS.cUa, Scer\GAL4^hh.PU

(Schleede and Blair, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The disrupted formation of the presumptive posterior crossvein (assessed by p-Mad staining) in the pupal wing observed in animals expressing Gyc76C^VDRC.cUa under the control of Scer\GAL4^hh.PU is not rescued by co-expression of Mmp1^{dsRNA.Scer\UAS}.

(Schleede and Blair, 2015)

Expression of Mmp1^{dsRNA.Scer\UAS} in enteroblasts under the control of Scer\GAL4^Su(H).GBE does not reduce tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

(Zhai et al., 2015)

Expression Mmp1^{dsRNA.Scer\UAS} in Scer\GAL4^Act5C.PI>Ras85D^V12.Scer\UAS, scrib¹ eye/antennal imaginal disc clones significantly suppresses the cell invasiveness phenotype but does not suppress the formation of tumors within the disc. Coexpression of Mmp1^{dsRNA.Scer\UAS} and Mmp2^{dsRNA.Scer\UAS} further reduces the invasiveness of Scer\GAL4^Act5C.PI>Ras85D^V12.Scer\UAS, scrib¹ cells.

(Uhlirova and Bohmann, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Expression of P{UAS-Mmp1.RNAi} produces dsRNA which results in dsRNA interference (RNAi) of the Mmp1 gene.

(Uhlirova and Bohmann, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mmp1^RNAi.UAS

Mmp1^{dsRNA.Scer\UAS}

Mmp1^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

Open Close