FlyBase Allele Report: Dmel\Dyb[11]

General Information

Symbol

Dmel\Dyb¹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0340166

Feature type

allele

Associated gene

Dmel\Dyb

Associated Insertion(s)

TI{TI}Dyb¹¹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Jantrapirom et al., 2019)

Mutagen

ends-out gene targeting

(Jantrapirom et al., 2019)

Progenitor genotype

Associated Insertion(s)

TI{TI}Dyb¹¹

Cytology

Description

Part of the Dyb locus, including the translation start codon used by 5 out of the 7 isoforms and the region encoding the EF-hand and ZZ domains common to all isoforms, has been deleted and replaced by a w^+mW.hs marker.

(Jantrapirom et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:12,446,314..12,449,720 [+]

Comment:

Curator inferred boundaries of a deletion resulting from homologous recombination in which the Dyb ATG and part of the coding region is removed and replaced with a w^+mW.hs marker.

(Jantrapirom et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | third instar larval stage

(Jantrapirom et al., 2019)

viable

(Jantrapirom et al., 2019)

Phenotype Manifest In

A1-7 ventral longitudinal muscle cell | larval stage

(Jantrapirom et al., 2019)

embryonic/larval hypodermal muscle cell | third instar larval stage

(Jantrapirom et al., 2019)

NMJ bouton | increased number | third instar larval stage

(Jantrapirom et al., 2019)

subsynaptic reticulum | larval stage

(Jantrapirom et al., 2019)

Detailed Description

Statement

Reference

Dyb¹¹ larvae exhibit a significant increase in the apparent patches within the SSR of Type Ib NMJ boutons, in the NMJ bouton number and in the number of nc82+ punctae per total synapses on body wall muscles 6 and 7 compared to controls.

Dyb¹¹ heterozygous or homozygous third instar larval NMJs exhibit a significant increase in evoked junctional potential (EJC) amplitudes and mean quantal content compared to controls. Also Dyb¹¹ third instar larval muscles 6 exhibit a significant increase in the cumulative EJC amplitude compared to controls.

(Jantrapirom et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dyb¹¹ has abnormal neurophysiology | third instar larval stage phenotype, suppressible by Cdc42⁴

(Jantrapirom et al., 2019)

Dyb¹¹ has abnormal neurophysiology | third instar larval stage phenotype, suppressible by Cdc42^KK108698/Scer\GAL4^Mhc.PU

(Jantrapirom et al., 2019)

Enhancer of

Statement

Reference

Dyb¹¹ is an enhancer of visible | adult stage phenotype of Dys^E6

(Jantrapirom et al., 2019)

Dyb¹¹ is an enhancer of visible | adult stage phenotype of cv-c¹

(Jantrapirom et al., 2019)

Other

Statement

Reference

Cdc42^KK108698, Dyb¹¹, Scer\GAL4^Mhc.PU has abnormal neurophysiology | third instar larval stage phenotype

(Jantrapirom et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dyb¹¹ has A1-7 ventral longitudinal muscle cell | larval stage phenotype, suppressible by Cdc42^KK108698/Scer\GAL4^Mhc.PU

(Jantrapirom et al., 2019)

Dyb¹¹ has A1-7 ventral longitudinal muscle cell | larval stage phenotype, suppressible by Cdc42⁴

(Jantrapirom et al., 2019)

Enhancer of

Statement

Reference

Dyb¹¹ is an enhancer of posterior crossvein phenotype of Dys^E6

(Jantrapirom et al., 2019)

Dyb¹¹ is an enhancer of wing phenotype of Dys^E6

(Jantrapirom et al., 2019)

Dyb¹¹ is an enhancer of posterior crossvein phenotype of cv-c¹

(Jantrapirom et al., 2019)

Dyb¹¹ is an enhancer of wing phenotype of cv-c¹

(Jantrapirom et al., 2019)

Other

Statement

Reference

Cdc42^KK108698, Dyb¹¹, Scer\GAL4^Mhc.PU has A1-7 ventral longitudinal muscle cell | larval stage phenotype

(Jantrapirom et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Dyb¹¹ heterozygosity or homozygosity enhances the posterior crossvein phenotype of cv-c¹ or Dys^E6 heterozygous adults.

Cdc42⁴ heterozygosity or expression of Cdc42^KK108698 under the control of Scer\GAL4^Mhc.PU suppresses the quantal content and evoked junctional potential phenotypes of Dyb¹¹ heterozygotes.

Expression of Cdc42^KK108698 under the control of Scer\GAL4^Mhc.PU leads to a significant increase in the frequency of miniature evoked junctional potentials in Dyb¹¹ heterozygous third instar larval muscle 6 compared to controls.

(Jantrapirom et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Dyb¹¹ is rescued by Scer\GAL4^how-24B/Dyb^UAS.Tag:MYC

(Jantrapirom et al., 2019)

Not rescued by

Dyb¹¹ is not rescued by Scer\GAL4^RapGAP1-OK6/Dyb^UAS.Tag:MYC

(Jantrapirom et al., 2019)

Comments