A TI{CRIMIC.TG4.1} DNA cassette has been inserted into prom, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The insertion may also affect the CG42383 gene, since it is only 57 bp upstream of the annotated 5' end. The TI{CRIMIC.TG4.1} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: TAGGCATTAAAAATGCATGTTGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.1} cassette is predicted to be accompanied by a deletion of 26bp of genomic sequence flanking the insertion site.