FlyBase Allele Report: Dmel\hob[2]

General Information

Symbol

Dmel\hob²

Species

D. melanogaster

Name

FlyBase ID

FBal0340793

Feature type

allele

Associated gene

Dmel\hob

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Neuman and Bashirullah, 2018)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q1392term.

(Neuman and Bashirullah, 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:3,214,209..3,214,209 [-]

Nucleotide change:

C3214209T

Amino acid change:

Q1392term | hob-PB; Q1392term | hob-PC

Reported amino acid change:

Q1392term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Neuman and Bashirullah, 2018)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with hob³)

(Neuman and Bashirullah, 2018)

abnormal size | third instar larval stage (with hob³)

(Neuman and Bashirullah, 2018)

decreased body size | pupal stage (with hob¹)

(Neuman and Bashirullah, 2018)

decreased body size | pupal stage (with hob³)

(Neuman et al., 2022, Neuman and Bashirullah, 2018)

decreased body size | pupal stage (with hob⁴)

(Neuman and Bashirullah, 2018)

decreased body size | pupal stage (with hob⁵)

(Neuman and Bashirullah, 2018)

lethal - all die before end of P-stage (with hob³)

(Neuman et al., 2022)

lethal - all die before end of P-stage (with hob⁴)

(Neuman and Bashirullah, 2018)

lethal - all die before end of P-stage (with hob⁵)

(Neuman and Bashirullah, 2018)

lethal - all die before end of pupal stage (with hob¹)

(Neuman and Bashirullah, 2018)

lethal - all die before end of pupal stage (with hob³)

(Neuman and Bashirullah, 2018)

viable (with hob³), with Scer\GAL4^Act.PU, hob^UAS.mCherry

(Neuman et al., 2022)

Phenotype Manifest In

late endosome | third instar larval stage (with hob³)

(Neuman and Bashirullah, 2018)

salivary gland (with hob³)

(Neuman and Bashirullah, 2018)

secretory granule | third instar larval stage (with hob³)

(Neuman and Bashirullah, 2018)

wing disc (with hob¹)

(Neuman and Bashirullah, 2018)

Detailed Description

Statement

Reference

The wing discs of hob¹/hob² transheterozygotes are smaller than in controls.

hob² homozygous clones within mosaic wing discs and salivary glands have a similar size as neighboring wild-type clones.

hob²/hob³ transheterozygous salivary glands display numerous enlarged (Rab7-positive) late endosomes and significantly smaller Sp33 secretory granules at the third instar larval stage; these also display impaired insulin secretion (Dilp5) and Sps3 secretion at the onset of puparium formation, as compared to controls; hob² homozygous clones within mosaic salivary glands display impaired Sps3 secretion at the onset of puparium formation, as compared to controls.

(Neuman and Bashirullah, 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

hob³/hob² has decreased body size | pupal stage phenotype, suppressible by Scer\GAL4^Act.PU/Hsap\BLTP2^UAS.cNa

(Neuman and Bashirullah, 2018)

hob³/hob² has lethal - all die before end of pupal stage phenotype, suppressible by Scer\GAL4^Act.PU/Hsap\BLTP2^UAS.cNa