FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4Piezo-KI
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General Information
Symbol
Scer\GAL4Piezo-KI
Species
S. cerevisiae
Name
FlyBase ID
FBal0340803
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
TI{GAL4}PiezoKI
Carried in Construct
Also Known As
Piezo-GAL4
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(He et al., 2018)
gene targeting by homologous recombination
(He et al., 2018)
Progenitor genotype
Associated Insertion(s)
TI{GAL4}PiezoKI
Cytology
Description

Sequence encoding the GAL4 open reading frame (with an ATG codon) followed by an SV40 polyadenylation signal has been inserted into Piezo, immediately after the first start codon (the 10 predicted Piezo isoforms share the same N-terminus). This results in the GAL4 driver being expressed under the control of the endogenous Piezo regulatory sequences, while expression of Piezo mRNA in homozygotes is reduced by approximately 50% compared to heterozygous controls (whose expression levels are not significantly different from wild type).

(He et al., 2018)
Allele components
Component
Use(s)
Inserted element
TI{GAL4}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The acute inhibition of Piezo-positive neurons, by expressing shi1.UAS under the control of Scer\GAL4Piezo-KI and testing adults at the non-permissive 32[o]C) increases feeding (also leading to gut bloating and increased crop size). Conversely, acute activation of Piezo-positive neurons, by expressing TrpA1UAS.(B).cKa under the control of Scer\GAL4Piezo-KI and activating it 30[o]C suppresses feeding (and crop size).

      (Min et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PiezoGal4.KI
      (Schoofs et al., 2024)
      Scer\GAL4Piezo-KI
      Name Synonyms
      Secondary FlyBase IDs
        References (8)