FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\mengMI03008-GFSTF.1
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General Information
Symbol
Dmel\mengMI03008-GFSTF.1
Species
D. melanogaster
Name
FlyBase ID
FBal0340831
Feature type
allele
Associated gene
Dmel\meng
Associated Insertion(s)
Mi{PT-GFSTF.1}mengMI03008-GFSTF.1
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Lee et al., 2018, Gene Disruption Project members, 2015-)
Progenitor genotype
Mi{MIC}mengMI03008
(Lee et al., 2018, Gene Disruption Project members, 2015-)
Associated Insertion(s)
Mi{PT-GFSTF.1}mengMI03008-GFSTF.1
Cytology
Description

The Mi{MIC} RMCE cassette in Mi{MIC}mengMI03008 is replaced with a multi-tag cassette (linker-EGFP-FIAsH-StrepII-3xFlag-linker) in-frame with the meng coding sequence, resulting in expression of an internally-tagged meng-EGFP-meng fusion protein.

(Lee et al., 2018)

The RMCE cassette of the Mi{MIC}CG11221MI03008 insertion has been replaced with a EGFP-FlAsH-StrepII-TEV-3xFlag multi-tag cassette. The insertion is in a coding intron of CG11221 and is predicted to protein trap the gene. The insertion is also within CG44271, not in a coding intron, and is not predicted to protein trap this gene.

(Gene Disruption Project members, 2015-)
Allele components
Component
Use(s)
Inserted element
Mi{PT-GFSTF.1}
Tagged with
EGFP
Tag:CALI(TC)
Tag:CS(TEVp)
Tag:FLAG
Tag:StrepII
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      mengMI03008-GFSTF.1 do not show defects in lifespan or in olfactory learning/memory, as compared to controls. Adults depleted of mengMI03008-GFSTF.1 in the mushroom body by the expression of slmbUAS.vhhGFP4 under the control of Scer\GAL4Mef2.247 for 3 days show a significant decrease in 3h and 24h olfactory memory, but not in olfactory learning, upon an olfactory aversive conditioning assay, as compared to controls; however, there are no effects in residual olfactory memory upon cold-shock, as compared to controls.

      (Lee et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG11221MI03008-GFSTF.1
      mengMI03008-GFSTF.1
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0340443
      • FBal0314732
      References (6)