A TI{CRIMIC.TG4.0} DNA cassette has been inserted into esc, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The insertion is also within Pde1c, in a coding intron, but is not in the correct reading frame to express GAL4 in the Pde1c pattern. The TI{CRIMIC.TG4.0} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: ATCGTTACAGGCTTTCAGCATGG. The homology arms of the donor vector overlapped by 1 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.