FlyBase Allele Report: Dmel\Yif1[10-46]

General Information

Symbol

Dmel\Yif1^10-46

Species

D. melanogaster

Name

FlyBase ID

FBal0342883

Feature type

allele

Associated gene

Dmel\Yif1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Wang et al., 2018)

Mutagen

ethyl methanesulfonate

(Wang et al., 2018)

Progenitor genotype

Cytology

Description

Nonsense mutation results in a truncated protein with the first 71 deduced amino acids.

(Wang et al., 2018)

Nucleotide substitution: C?T.

(Wang et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:26,718,522..26,718,522 [+]

Nucleotide change:

C26718522T

Reported nucleotide change:

C?T

Amino acid change:

Q77term | Yif1-PA; Q73term | Yif1-PB; Q82term | Yif1-PC

Reported amino acid change:

Q72term

(Wang et al., 2018)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | pupal stage | somatic clone

(Wang et al., 2018)

abnormal neuroanatomy | somatic clone | wandering third instar larval stage

(Wang et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Yif1^10-46 mutant ddaC neuron clones show significantly simplified dendrite arbors with fewer dendritic termini in wandering third instar larvae and show strong pruning defects in pupae (16h APF); mutant ddaD/E neuron clones also show strong pruning defects in pupae (19h APF); mutant ddaF neuron clones are removed by 19h APF, similar to wild-type controls.

Yif1^10-46 mutant ddaC neuron clones in wandering third instar larvae show disrupted cis- and trans-Golgi (as shown by the diffuse patterns of fluorescent GM130, Arf1 and ManII) and disrupted secretory vesicles (as shown by the diffuse pattern of fluorescent Sec15), but no defect in ER exit sites (as shown by the normal punctate pattern of fluorescent Sec31).

(Wang et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Yif1^10-46 has abnormal neuroanatomy | somatic clone | pupal stage phenotype, suppressible by Nrg^GL00656/Scer\GAL4^ppk.PU

(Wang et al., 2018)

Yif1^10-46 has abnormal neuroanatomy | somatic clone | pupal stage phenotype, suppressible by Nrg^HMS01638/Scer\GAL4^ppk.PU