UASt regulatory sequences drive expression of yki cDNA (isoform F) that contains an in-frame deletion of 47 amino acids (aa 35-82), tagged with Tag:Myr(Src64B) and 3xTag:FLAG at the N-terminus.
Adults expressing ykiΔNH.UAS.Tag:FLAG,Tag:Myr(Src64B) under the control of Scer\GAL4nub-AC-62 display significantly bigger wings, as compared to controls.Wing discs expressing ykiΔNH.UAS.Tag:FLAG,Tag:Myr(Src64B) under the control of Scer\GAL4ap-md544 show an indentation at the wing disc epithelium dorsal-ventral boundary, as compared to controls.
Wing disc clones expressing ykiΔNH.UAS.Tag:FLAG,Tag:Myr(Src64B) under the control of Scer\GAL4Tub.PU occupy a significantly larger disc area than control clones.
ykiΔNH.UAS.Tag:FLAG,Tag:Myr(Src64B)/Scer\GAL4Tub.PU fails to rescue ykiB5