FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\SAKAa74
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General Information
Symbol
Dmel\SAKAa74
Species
D. melanogaster
Name
FlyBase ID
FBal0343976
Feature type
allele
Associated gene
Dmel\SAK
Associated Insertion(s)
Carried in Construct
Also Known As
Plk4Aa74
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Aydogan et al., 2018)
Mutagen
P-element activity
(Aydogan et al., 2018)
Progenitor genotype
P{GSV1}GS3043
(Aydogan et al., 2018)
Caused by aberration
Df(3L)SAKAa74
Cytology
Description

1751bp deletion that removes the first third of the SAK coding sequence (including all sequence encoding the kinase domain). Some Cdk12 5'UTR sequence is also deleted (the site of the original P{GSV1}GS3043 insertion is within the Cdk12 5'UTR).

(Aydogan et al., 2018)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:21,514,997..21,516,747 [-]
Comment:

Inferred boundaries of a 1751bp deletion resulting from the imprecise excision of P{GSV1}GS3043. The P{GSV1}GS3043 insertion site is reported to be 996bp upstream of the SAK ATG and the deletion extends in one direction toward SAK from the insertion site.

(Aydogan et al., 2018)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      SAKAa74 homozygous adults are uncoordinated and fail to perform in climbing assay (negative gravitaxis assay) when compared to controls.

      SAKAa74 homozygous third instar larval brains lack centrioles (GFP-PACT staining) when compared to controls.

      SAKAa74/+ embryos do not exhibit any centriole size changes, centriole duplication defects or changes in hatching rates when compared to controls.

      SAKAa74/+ embryos exhibit centrioles with a significantly reduced growth rate and a significantly increased growth period compared to controls.

      (Aydogan et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      SAKAa74 is rescued by SAKUbi.GFP

      (Aydogan et al., 2018)
      Partially rescued by

      SAKAa74 is partially rescued by SAKUAS.SAK.EGFP

      (Aydogan et al., 2018)
      Comments

      Expression SAKUbi.GFP/+ rescues the uncoordinated and no centriole phenotypes of SAKAa74 homozygous adults.

      Expression SAKUAS.SAK.EGFP/+ partially rescues the climbing defect and uncoordinated phenotype of SAKAa74 homozygous adults.

      (Aydogan et al., 2018)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Plk4Aa74
      (Wong et al., 2025, Aydogan et al., 2022, Steinacker et al., 2022, Aydogan et al., 2020, Aydogan et al., 2018)
      Plk4aa74
      (Gartenmann et al., 2020)
      SAKAa74
      Name Synonyms
      Secondary FlyBase IDs
        References (6)