FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\cacSL.UAS.EGFP
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General Information
Symbol
Dmel\cacSL.UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0344040
Feature type
allele
Associated gene
Dmel\cac
Associated Insertion(s)
Carried in Construct
P{UAS-cac.SL.EGFP}
Key Links
Genomic Maps

Transgenic product class
missense_variant
(Inagaki et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Inagaki et al., 2014)
Mutagen
in vitro construct
(Inagaki et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-cac.SL.EGFP}
Cytology
Description

UASt regulatory sequences drive expression of cac bearing a a serine 161 to leucine substitution corresponding to S218L in the human CACNA1A gene (a mutation associated with familial hemiplegic migraine). The protein is tagged with EGFP.

(Inagaki et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
cac
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:11,977,023..11,977,024 [-]
Nucleotide change:

TC11977024CT

Amino acid change:

S161L | cac-PA; S161L | cac-PB; S161L | cac-PC; S161L | cac-PD; S161L | cac-PE; S161L | cac-PF; S161L | cac-PG; S161L | cac-PH; S161L | cac-PI; S161L | cac-PJ; S161L | cac-PL; S161L | cac-PM; S267L | cac-PN; S161L | cac-PO; S161L | cac-PP; S161L | cac-PS; S161L | cac-PT; S161L | cac-PU

Reported amino acid change:

S161L

Comment:

Analogous S218L mutation in human CACNA1A implicated in migraine, familial hemiplegic 1; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.

(Brusich et al., 2018)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  familial hemiplegic migraine 1
      CEA
      (Brusich et al., 2018, Inagaki et al., 2014)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Inagaki et al., 2014)
      CACNA1A:p.Ser218Leu
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: CACNA1A_8504
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Early third instar larvae expressing cacSL.UAS.EGFP under the control of Scer\GAL4elav-C155 develop protruding anterior spiracles prematurely, which normally only develop at wandering third instar stage and pupation. Their decreased adult viability is more severe in males than in females. In CNS-removed larvae, NMJ neurotransmission shows a significant increase in mEPSP frequency, but not amplitude or rise time, and a significant increase in EPSP amplitude, but not quantal content, as compared to controls; larger spontaneous events are slower and there is rare gigantic spontaneous events, which are never observed in controls. Upon 30 presynaptic pulses, EPSP profiles exhibit shoulders and extra discharges.

      (Brusich et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4elav-C155/cacSL.UAS.EGFP fails to rescue cacHC129

      (Brusich et al., 2018)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      cacSL.UAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)