FB2026_02 , released June 18, 2026
Allele: Dmel\cacRQ.UAS.EGFP
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General Information
Symbol
Dmel\cacRQ.UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0344041
Feature type
allele
Associated gene
Dmel\cac
Associated Insertion(s)
Carried in Construct
P{UAS-cac.RQ.EGFP}
Key Links
Genomic Maps

Transgenic product class
missense_variant
(Brusich et al., 2018)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Brusich et al., 2018)
Mutagen
in vitro construct
(Brusich et al., 2018)
Progenitor genotype
Carried in construct
P{UAS-cac.RQ.EGFP}
Cytology
Description

UASt regulates expression of EGFP-tagged cac bearing an arginine 135 to glutamine substitution corresponding to R192Q in mammalian CACNA1A.

(Brusich et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
cac
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:11,977,518..11,977,519 [-]
Nucleotide change:

AG11977519CA

Amino acid change:

R135Q | cac-PA; R135Q | cac-PC; R135Q | cac-PE; R135Q | cac-PH; R135Q | cac-PI; R135Q | cac-PL; R135Q | cac-PM; R241Q | cac-PN; R135Q | cac-PO; R135Q | cac-PP; R135Q | cac-PS; R135Q | cac-PT; R135Q | cac-PU

Reported amino acid change:

R135Q

Comment:

Analogous R192Q mutation in human CACNA1A implicated in migraine, familial hemiplegic 1; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.

(Brusich et al., 2018)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Brusich et al., 2018)
      CACNA1A:p.Arg192Gln
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: CACNA1A_8487
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In CNS-removed larvae expressing cacRQ.UAS.EGFP under the control of Scer\GAL4elav-C155, NMJ neurotransmission does not show significant changes in mEPSP frequency, amplitude and rise time, or in EPSP amplitude and quantal content; larger spontaneous events are slower; there are no gigantic spontaneous events. Upon 30 presynaptic pulses, EPSP profiles exhibit shoulders but no extra discharges; EPSP shoulders are observed both in otherwise wild-type and cacHC129 mutant backgrounds.

      (Brusich et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      cacRQ.UAS.EGFP/Scer\GAL4elav-C155 partially rescues cacHC129

      (Brusich et al., 2018)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      cacRQ.UAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)