FlyBase Allele Report: Hsap\RELA[AD.R23H06]

General Information

Symbol

Hsap\RELA^AD.R23H06

Species

H. sapiens

Name

FlyBase ID

FBal0344100

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R23H06-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(O'Sullivan et al., 2018)

Progenitor genotype

Carried in construct

P{R23H06-p65.AD}

Cytology

Description

The GMR23H06 enhancer fragment is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(O'Sullivan et al., 2018)

Allele components

Component

Use(s)

Regulatory region(s)

GMR23H06

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

DLM motor neuron

Comment: when combined with P{R30A07-GAL4.DBD} (combination referred to as 'dlm-SG')

(O'Sullivan et al., 2018)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R30A07

(O'Sullivan et al., 2018)

abnormal song, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R30A07

(O'Sullivan et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Silencing (using Ctet\TeTxLC^TNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R30A07 and Hsap\RELA^AD.R23H06 in adult males significantly alters some parameters of pulse song, but not sine song, compared to controls; there is no significant effect on the number of males that sing, wing extension toward females, or copulation success, compared to controls; take off and forced flight ability are significantly impaired compared to controls.

(O'Sullivan et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions