FlyBase Allele Report: Scer\GAL4[DBD.R30A07]

General Information

Symbol

Scer\GAL4^DBD.R30A07

Species

S. cerevisiae

Name

FlyBase ID

FBal0331650

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R30A07-GAL4.DBD}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R30A07-GAL4.DBD}

Cytology

Description

The GMR30A07 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR30A07

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

DLM motor neuron

Comment: when combined with P{R23H06-p65.AD} (combination referred to as 'dlm-SG')

(O'Sullivan et al., 2018)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R23H06

(O'Sullivan et al., 2018)

abnormal song, with Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R23H06

(O'Sullivan et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Silencing (using Ctet\TeTxLC^TNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R30A07 and Hsap\RELA^AD.R23H06 in adult males significantly alters some parameters of pulse song, but not sine song, compared to controls; there is no significant effect on the number of males that sing, wing extension toward females, or copulation success, compared to controls; take off and forced flight ability are significantly impaired compared to controls.

(O'Sullivan et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions