FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\unc-104AAA.UAS.Tag:HA
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General Information
Symbol
Dmel\unc-104AAA.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0344773
Feature type
allele
Associated gene
Dmel\unc-104
Associated Insertion(s)
Carried in Construct
P{UAS-unc-104.AAA}
Key Links
Transgenic product class
dominant_negative_variant
(Zong et al., 2018)
missense_variant
(Zong et al., 2018)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Zong et al., 2018)
missense_variant
(Zong et al., 2018)
Mutagen
in vitro construct
(Zong et al., 2018)
Progenitor genotype
Carried in construct
P{UAS-unc-104.AAA}
Cytology
Description

UASt regulatory sequences drive expression of a dominant-negative form of unc-104 in which the ATP-binding sequence of the motor domain has been mutated from GQTGAGKS to GQTGAAAA. The coding sequence is tagged at the C-terminal end with Tag:HA.

(Zong et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
unc-104
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of unc-104AAA.UAS.Tag:HA under the control of Scer\GAL4GSG2295 and induced by RU486 feeding results in mild-to-severe and highly fully penetrant ddaC neuron dendrite pruning defects at 16h after puparium formation, as compared to wild-type or non-induced controls. The same is observed upon Scer\GAL4ppk.PU-driven expression.

      (Zong et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      unc-104AAA.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)