FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\sdtΔ3-GFP
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General Information
Symbol
Dmel\sdtΔ3-GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0345003
Feature type
allele
Associated gene
Dmel\sdt
Associated Insertion(s)
TI{TI}sdtΔ3-GFP
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Perez-Mockus et al., 2017)
Mutagen
gene targeting by homologous recombination
(Perez-Mockus et al., 2017)
CRISPR/Cas9
(Perez-Mockus et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}sdtΔ3-GFP
Cytology
Description

Exon 3 of endogenous sdt is replaced by in frame sfGFP. This results in sfGFP-tagged proteins lacking the sequence of exon 3, hence lacking the neur binding motif.

(Perez-Mockus et al., 2017)
Allele components
Component
Use(s)
Tagged with
sfGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
X:8,224,785..8,226,083 [+]
Comment:

This alternatively spliced exon is replaced in-frame with sfGFP.

(Perez-Mockus et al., 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Stage 9/10 sdtΔ3-GFP embryos do not show any obvious defects in epithelial integrity or apical-basal cell polarity (assessed by the localization of aPKC and Nrt proteins). Stage 10 embryos show defects in posterior midgut remodeling, as shown by significantly fewer PGCs migrating through the posterior midgut epithelium, as compared to controls. In mutant eye discs, about half of ommatidia fail to form the expected six-cell rosettes and instead cells form elongated structures favoring early R3/R4 contacts, In adults, about half of ommatidia fail to form a rosette.

      (Perez-Mockus et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Stage 9/10 Df(3L)Brd-C1/Df(3R)E(spl)δ-6 transheterozygous embryos display loss of apical-basal cell polarity and of epithelial integrity; these are rescued by sdtΔ3-GFP.

      Stage 9/10 Df(3L)Brd-C1/Df(3R)E(spl)δ-6 transheterozygous embryos display loss of apical-basal cell polarity and of epithelial integrity; both of these defects are rescued by sdtΔ3-GFP.

      (Perez-Mockus et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sdtΔ3-GFP
      (Perez-Mockus et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)