FlyBase Allele Report: Dmel\hts[R913fs]

General Information

Symbol

Dmel\hts^R913fs

Species

D. melanogaster

Name

FlyBase ID

FBal0345601

Feature type

allele

Associated gene

Dmel\hts

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Hudson et al., 2019)

gene targeting by homologous recombination

(Hudson et al., 2019)

Progenitor genotype

Cytology

Description

559bp deletion ( 2R:19400036..19400594 , release 6 genome) in hts exon 12, resulting in a frameshift extension of 10 amino acids beginning at R913, immediately prior to the DRERPEAEQ sequence.

(Hudson et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:19,400,036..19,400,594 [-]

Comment:

A 559bp deletion ( 2R:19400036..19400594 ) leads to a frameshift at amino acid 913 of isoforms hts-PA, hts-PK.

(Hudson et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg

(Hudson et al., 2019)

filamentous actin | oogenesis

(Hudson et al., 2019)

filamentous actin | oogenesis (with Df(2R)BSC135)

(Hudson et al., 2019)

nurse cell ring canal

(Hudson et al., 2019)

nurse cell ring canal (with Df(2R)BSC135)

(Hudson et al., 2019)

oocyte

(Gerdes et al., 2020)

Detailed Description

Statement

Reference

hts^R913fs homozygotes and hts^I775fs/Df(2R)BSC135 transheterozygotes do not have fusome defects but egg chamber ring canals lack the robust F-actin inner rim observed in wild-type controls. Eggs laid are significantly smaller than controls.

(Hudson et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

hts^R913fs has decreased cell size phenotype, non-enhanceable by kel^DE1/kel^DE1

(Hudson et al., 2019)

NOT suppressed by

Statement

Reference

hts^R913fs has decreased cell size phenotype, non-suppressible by kel^DE1/kel^DE1

(Hudson et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

hts^R913fs has egg phenotype, non-enhanceable by kel^DE1/kel^DE1

(Hudson et al., 2019)

NOT suppressed by

Statement

Reference

hts^R913fs has nurse cell ring canal phenotype, non-suppressible by kel^DE1/kel^DE1

(Hudson et al., 2019)

hts^R913fs has filamentous actin | oogenesis phenotype, non-suppressible by kel^DE1/kel^DE1

(Hudson et al., 2019)

hts^R913fs has egg phenotype, non-suppressible by kel^DE1/kel^DE1

(Hudson et al., 2019)

Suppressor of

Statement

Reference

hts^R913fs/hts^R913fs is a suppressor of nurse cell ring canal phenotype of kel^DE1

(Hudson et al., 2019)

hts^R913fs/hts^R913fs is a suppressor of filamentous actin | oogenesis phenotype of kel^DE1

(Hudson et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

kel^DE1, hts^R913fs double homozygotes exhibit the same thinner egg chamber ring canal F-actin rim phenotype observed in hts^R913fs single homozygotes rather than the very thick F-actin rim phenotype observed in kel^DE1 single homozygotes.

(Hudson et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

hts^R913fs

(Gerdes et al., 2020, Hudson et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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