FlyBase Allele Report: Dmel\hts[I775fs]

General Information

Symbol

Dmel\hts^I775fs

Species

D. melanogaster

Name

FlyBase ID

FBal0345602

Feature type

allele

Associated gene

Dmel\hts

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Hudson et al., 2019)

gene targeting by homologous recombination

(Hudson et al., 2019)

Progenitor genotype

Cytology

Description

2bp deletion ( 2R:19401008..19401009 , release 6 genome) in hts exon 12, predicted to result in a 62 amino acid extension in the new reading frame starting at I775 of Ovhts.

(Hudson et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:19,401,008..19,401,009 [-]

Comment:

A two base pair deletion ( 2R:19401008..19401009 ) leads to a frameshift at amino acid I775 of isoforms hts-PA, hts-PK, and hts-PN. 62 novel amino acids are added before a premature stop codon is reached.

(Hudson et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased fecundity | female

(Gerdes et al., 2020)

decreased fecundity | female (with Df(2R)BSC135)

(Gerdes et al., 2020)

decreased size | female

(Gerdes et al., 2020)

female semi-fertile (with Df(2R)BSC135)

(Gerdes et al., 2020)

majority die during embryonic stage | maternal effect (with Df(2R)BSC135)

(Gerdes et al., 2020)

partially lethal - majority die | maternal effect (with Df(2R)BSC135)

(Gerdes et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

hts^I775fs homozygotes and hts^I775fs/Df(2R)BSC135 transheterozygotes do not have fusome defects but egg chamber ring canals lack the robust F-actin inner rim observed in wild-type controls.

(Hudson et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

hts^I775fs is rescued by hts^{Ovhts.otu.GFP(S65T)}

(Hudson et al., 2019)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

hts^I775fs

(Gerdes et al., 2020, Hudson et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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