FlyBase Allele Report: Dmel\hts[T776fs]

General Information

Symbol

Dmel\hts^T776fs

Species

D. melanogaster

Name

FlyBase ID

FBal0345603

Feature type

allele

Associated gene

Dmel\hts

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Hudson et al., 2019)

gene targeting by homologous recombination

(Hudson et al., 2019)

Progenitor genotype

Cytology

Description

1bp deletion ( 2R:19401007 , release 6 genome) in hts exon 12, resulting in a frameshift extension of 28 amino acids beginning at T776.

(Hudson et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:19,401,007..19,401,007 [-]

Comment:

A one base pair deletion (T19401007) leads to a frameshift at amino acid T776 of isoforms hts-PA, hts-PK, and hts-PN. 28 novel amino acids are added before a premature stop codon is reached.

(Hudson et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

filamentous actin | oogenesis

(Hudson et al., 2019)

filamentous actin | oogenesis (with Df(2R)BSC135)

(Hudson et al., 2019)

nurse cell ring canal

(Hudson et al., 2019)

nurse cell ring canal (with Df(2R)BSC135)

(Hudson et al., 2019)

Detailed Description

Statement

Reference

hts^T776fs homozygotes and hts^I775fs/Df(2R)BSC135 transheterozygotes do not have fusome defects but egg chamber ring canals lack the robust F-actin inner rim observed in heterozygous and wild-type controls.

(Hudson et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

hts[+]/hts^T776fs is a suppressor of nurse cell ring canal phenotype of Cul3^HMS01572, Scer\GAL4^{VP16.mat.αTub67C}

(Hudson et al., 2019)

hts[+]/hts^T776fs is a suppressor of filamentous actin | oogenesis phenotype of Cul3^HMS01572, Scer\GAL4^{VP16.mat.αTub67C}

(Hudson et al., 2019)

hts[+]/hts^T776fs is a suppressor of nurse cell ring canal phenotype of Prosβ5^HMS00119, Scer\GAL4^{VP16.mat.αTub67C}

(Hudson et al., 2019)

hts[+]/hts^T776fs is a suppressor of filamentous actin | oogenesis phenotype of Prosβ5^HMS00119, Scer\GAL4^{VP16.mat.αTub67C}

(Hudson et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

hts^T776fs

(Hudson et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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