FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\VAChT14Q
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General Information
Symbol
Dmel\VAChT14Q
Species
D. melanogaster
Name
FlyBase ID
FBal0346876
Feature type
allele
Associated gene
Dmel\VAChT
Associated Insertion(s)
TI{TI}VAChT14Q
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Vernon et al., 2019)
gene targeting by homologous recombination
(Vernon et al., 2019)
Progenitor genotype
Associated Insertion(s)
TI{TI}VAChT14Q
Cytology
Description

The polyQ region of the endogenous VAChT gene has been extended by one glutamine residue, to 14 residues. A loxP cassette containing a Disc\RFPDsRed(T1).3xP3.cHa marker has also been inserted into the genome as part of the mutagenesis procedure.

(Vernon et al., 2019)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      VAChT14Q homozygous late stage embryonic aCC/RP2 motoneurons exhibit significantly reduced amplitude and frequency of spontaneous mEPSC when compared to controls. Also they lack any observable evoked spontaneous rhythmic currents (SRCs) or optogenetically (using Crei\ChR2CHETA(E123T.H134R).UAS.YFP under the control of Scer\GAL4ChAT.BAC) evoked EPSCs different than controls.

      (Vernon et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      VAChT14Q homozygous late stage embryonic aCC/RP2 motoneurons lack any observable evoked spontaneous rhythmic currents (SRCs) or optogenetically (using Crei\ChR2CHETA(E123T.H134R).UAS.YFP under the control of Scer\GAL4ChAT.BAC) evoked EPSCs different than controls.

      (Vernon et al., 2019)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      VAChT14Q
      (Vernon et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)