FlyBase Allele Report: Dmel\PolD3[L30]

General Information

Symbol

Dmel\PolD3^L30

Species

D. melanogaster

Name

FlyBase ID

FBal0350363

Feature type

allele

Associated gene

Dmel\PolD3

Associated Insertion(s)

P{?EPgy2}PolD3^L30

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Ji et al., 2019)

Mutagen

P-element activity

(Ji et al., 2019)

Progenitor genotype

P{EPgy2}PolD3^EY15283

(Ji et al., 2019)

Associated Insertion(s)

P{?EPgy2}PolD3^L30

Cytology

Description

Mobilization of the progenitor P{EPgy2}Pol32^EY15283 insertion, resulting in deletion of nt15255502 to nt15256304 with an addition of 39bp of P element sequences. Since only part of the Pol32 coding region is removed, along with the entire annotated 3'UTR, there is still a possibility that a truncated Pol32 protein with 219 residues is produced, though this is undetectable by Western Blot analyses.

(Ji et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

complex_substitution

2L:15,255,502..15,256,304 [-]

Comment:

An 803 bp deletion resulting from the imprecise excision of P{EPgy2}Pol32^EY15283. Reported to map to 2L:15255502..15256304 . 39 bases of P element sequences remain at the site of the excision.

(Ji et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage

(Ji et al., 2019)

female sterile

(Ji et al., 2019)

lethal - all die during embryonic stage | maternal effect

(Ji et al., 2019)

male fertile

(Ji et al., 2019)

Phenotype Manifest In

chromosome | third instar larval stage (with PolD3^L27)

(Ji et al., 2019)

embryonic neuroblast | third instar larval stage (with PolD3^L27)

(Ji et al., 2019)

macrochaeta

(Ji et al., 2019)

Detailed Description

Statement

Reference

Pol32^L30 mutant adults express a variable degree of bristle loss or shortening, as compared to controls. Females are sterile, as they lay eggs that are fertilized but do not show more than 8 nuclei; in agreement, most of the DNA from these embryos is mitochondrial.

A small proportion of Pol32^L30/Pol32^L27 third instar larvae show double strand breaks, which are nearly absent in controls. A very small proportion of adults show an etched abdomen phenotype.

(Ji et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

PolD1^I10/DNApol-delta[+], PolD3^L30/PolD3^L27 has visible phenotype

(Ji et al., 2019)

Df(3L)BSC122/+, PolD3^L30/PolD3^L27 has visible phenotype

(Ji et al., 2019)

PolD2^G16501/Pol31[+], PolD3^L30/PolD3^L27 has visible phenotype

(Ji et al., 2019)

DNApol-alpha180[+]/PolA1^G13925, PolD3^L30/PolD3^L27 has visible phenotype

(Ji et al., 2019)

Df(3R)Exel6186/+, PolD3^L30/PolD3^L27 has visible phenotype

(Ji et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

PolD3^L30/PolD3^L27 has chromosome | third instar larval stage phenotype, enhanceable by PolD1^I10/DNApol-delta[+]

(Ji et al., 2019)

PolD3^L30/PolD3^L27 has embryonic neuroblast | third instar larval stage phenotype, enhanceable by PolD1^I10/DNApol-delta[+]

(Ji et al., 2019)

Other

Statement

Reference

PolD1^I10/DNApol-delta[+], PolD3^L30/PolD3^L27 has adult abdomen phenotype

(Ji et al., 2019)

Df(3L)BSC122/+, PolD3^L30/PolD3^L27 has adult abdomen phenotype

(Ji et al., 2019)

PolD2^G16501/Pol31[+], PolD3^L30/PolD3^L27 has adult abdomen phenotype

(Ji et al., 2019)

DNApol-alpha180[+]/PolA1^G13925, PolD3^L30/PolD3^L27 has adult abdomen phenotype

(Ji et al., 2019)

Df(3R)Exel6186/+, PolD3^L30/PolD3^L27 has adult abdomen phenotype

(Ji et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

A small proportion of Pol32^L30/Pol32^L27 third instar larvae show double strand breaks, both centric and non-centric, which are nearly absent in controls; these become much more frequent in a DNApol-δ^I10 heterozygous background and are both centric and non-centric.

A very small proportion of Pol32^L30/Pol32^L27 adults show an etched abdomen phenotype; this phenotype is only slightly increased in a DNApol-α180^G13925/+ background, is moderately increased in a Pol31^G16501/+ background, becomes highly prevalent in Df(3L)BSC122/+ or Df(3R)Exel6186/+ backgrounds and becomes nearly fully penetrant in a DNApol-δ^I10/+ background; the phenotype is not much affected in Df(3R)X3F/+, spn-A¹/+, Df(3R)P-21/+, or Blm^D2/+ backgrounds.