A TI{CRIMIC.TG4.1} DNA cassette has been inserted into DIP-ι, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.1} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: CGGAGTGGTAGCGTATAAAATGG. The homology arms of the donor plasmid overlapped by 22bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.
DIP-ιCR00997-TG4.1 is an enhancer of abnormal neurophysiology | adult stage phenotype of Hsap\MAPTP301L.QUAS.0N4R, Ncra\QFQF2w.nSyb
DIP-ιCR00997-TG4.1 is an enhancer of retina | adult stage phenotype of Hsap\MAPTP301L.QUAS.0N4R, Ncra\QFQF2w.nSyb