Insertion of TI{CRISPaint.T2A-GAL4} sequence into the 5' part of the Myo31DF coding sequence. The insertion is in sense orientation (T2A-GAL4 sequence within the inserted sequence cassette is in the same orientation as transcription of Myo31DF), but is out of frame with respect to the Myo31DF open reading frame. However, GAL4 expression is seen. An indel (4bp deletion, 2bp insertion) is associated with the site of the TI{CRISPaint.T2A-GAL4} insertion.
Insertion of TI{CRISPaint.T2A-GAL4} sequence into the 5' part of the Myo31DF coding sequence. The insertion is in sense orientation (T2A-Scer\GAL4 sequence within the inserted sequence cassette is in the same orientation as transcription of Myo31DF), but is out of frame with respect to the Myo31DF open reading frame. An indel (4bp deletion, 2bp insertion) is associated with the site of the TI{CRISPaint.T2A-GAL4} insertion.
TC