FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Bosch, J., Perrimon, N. (2019.9.30). CRISPaint T2A-GAL4 construct and insertions from Norbert Perrimon. 
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FBrf0243692
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Personal communication to FlyBase
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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Justin Bosch and Norbert Perrimon, Harvard Medical School.
TI{CRISPaint.T2A-GAL4}eFP545-2 is an insertion into ebony at approximately  3R:21232424..21232439 . Flies exhibit an ebony reduction of function phenotype and the insertion fails to complement e1. GAL4 expression is seen in anterior and posterior tracheae in larvae, in the larval brain, and in pupal and adult cuticle.
TI{CRISPaint.T2A-GAL4}MhcGP00583-1 is an insertion at approximately  2L:16774654..16774664 . The insertion fails to complement P{lacW}Mhck10423 and Df(2L)H20. GAL4 expression can be seen in larval muscle.
TI{CRISPaint.T2A-GAL4}Myo31DFGP00364-1 is an insertion at approximately  2L:10505998..10506002 . The insertions fails to complement Df(2L)ED8142.
GAL4 expression can be seen in larval gut.
TI{CRISPaint.T2A-GAL4}wgGP06461-1 is an insertion at approximately  2L:7307657..7307702 . The insertion fails to complement wgl-17, wgl-8, and Df(2L)BSC291. Strong GAL4 expression can be seen in wing imaginal discs.
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Related Publication(s)
Research paper

Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids.
Bosch et al., 2020, Genetics 214(1): 75--89 [FBrf0244507]

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