FlyBase Allele Report: Dmel\ccb[RNAi.UAS.cMa]

General Information

Symbol

Dmel\ccb^RNAi.UAS.cMa

Species

D. melanogaster

Name

UAS construct a of Martin-Pena

FlyBase ID

FBal0358590

Feature type

allele

Associated gene

Dmel\ccb

Associated Insertion(s)

Carried in Construct

P{UAS-ccb.RNAi.M}

Key Links

Transgenic product class

RNAi_reagent

(Martin-Peña and Ferrus, 2020)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Martin-Peña and Ferrus, 2020)

Mutagen

in vitro construct

(Martin-Peña and Ferrus, 2020)

Progenitor genotype

Carried in construct

P{UAS-ccb.RNAi.M}

Cytology

Description

UASt regulates expression of a construct containing a 778bp genomic ccb DNA following by a 665bp ccb cDNA (in reverse orientation), which generates a hairpin targeting ccb for RNAi-mediated degradation.

(Martin-Peña and Ferrus, 2020)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ccb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

partially lethal - majority die, with Scer\GAL4^elav.PU

(Martin-Peña and Ferrus, 2020)

Phenotype Manifest In

ellipsoid body, with Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

Detailed Description

Statement

Reference

Expression of ccb^{dsRNA.UAS.cMa} under the control of Scer\GAL4^Alp4-c507 leads to morphological defects in ellipsoid body in adults.

(Martin-Peña and Ferrus, 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507 is an enhancer of abnormal neuroanatomy | adult stage phenotype of Rab11^{S25N.UASp.YFP}, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

NOT Enhancer of

Statement

Reference

ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507 is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

NOT Suppressor of

Statement

Reference

ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

Other

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^Toll-6-D42, ccb^RNAi.UAS.cMa has abnormal neuroanatomy | larval stage phenotype

(Martin-Peña and Ferrus, 2020)

Rab11^UAS.EGFP, Scer\GAL4^Toll-6-D42, ccb^RNAi.UAS.cMa has decreased size | larval stage phenotype

(Martin-Peña and Ferrus, 2020)

Phenotype Manifest In

Enhancer of

Statement

Reference

ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507 is an enhancer of ellipsoid body phenotype of Rab11^{S25N.UASp.YFP}, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507 is an enhancer of adult ellipsoid body R-neuron phenotype of Rab11^{S25N.UASp.YFP}, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

NOT Enhancer of

Statement

Reference

ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507 is a non-enhancer of ellipsoid body phenotype of Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507 is a non-enhancer of adult ellipsoid body R-neuron phenotype of Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

NOT Suppressor of

Statement

Reference

ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507 is a non-suppressor of ellipsoid body phenotype of Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

ccb^RNAi.UAS.cMa, Scer\GAL4^Alp4-c507 is a non-suppressor of adult ellipsoid body R-neuron phenotype of Rab11^{Q70L.UASp.YFP}, Scer\GAL4^Alp4-c507

(Martin-Peña and Ferrus, 2020)

Other

Statement

Reference

Rab11^UAS.EGFP, Scer\GAL4^Toll-6-D42, ccb^RNAi.UAS.cMa has embryonic/larval neuromuscular junction phenotype

(Martin-Peña and Ferrus, 2020)

Rab11^UAS.EGFP, Scer\GAL4^Toll-6-D42, ccb^RNAi.UAS.cMa has A1-7 ventral longitudinal muscle cell | larval stage phenotype

(Martin-Peña and Ferrus, 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ccb^{dsRNA.UAS.cMa} and Rab11^UAS.EGFP under the control of Scer\GAL4^Toll-6-D42 leads to a decrease in the size of NMJ of larval motor neurons innervating muscle fibers 6 and 7.

(Martin-Peña and Ferrus, 2020)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ccb^RNAi.UAS.cMa

ccb^{dsRNA.UAS.cMa}

Name Synonyms

UAS construct a of Martin-Pena

Secondary FlyBase IDs

References (2)

Report Sections

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