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General Information
Symbol
Dmel\Rab11UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0156007
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Rab11-GFP, UAS-GFP-Rab11, Rab11-GFP, UASRab11-GFP, GFP-Rab11
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The Rab11 coding sequence is fused to EGFP; expression is governed by UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Rab11dsRNA.UAS.WIZ under the control of Scer\GAL4Ugt36A1.PK) leads to mild slit diagram localization defects.

Expression of Rab11Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Bx-MS1096 has no effect on wing development.

Sucrose dye fed to flies expressing Rab11Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Myo31DF-NP0001 leaks into the body cavity, indicating that the epithelial barrier is breached.

Expression of Rab11Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Myo31DF-NP0001 increases the survival of flies infected with Vibrio cholerae (both wild-type and &ctxa V. cholerae strains).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhancer of
Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of ccbdsRNA.UAS.cMa and Rab11UAS.EGFP under the control of Scer\GAL4Toll-6-D42 leads to a decrease in the size of NMJ of larval motor neurons innervating muscle fibers 6 and 7.

The increased average amplitude of miniature (in case of Hsap\SNCAScer\UAS.cFa) or evoked excitatory junction potentials (in case of Hsap\SNCAScer\UAS.cKa) at neuromuscular junctions of third instar larvae as well as the abnormal frequency distribution of mEJP amplitudes seen in larvae expressing either Hsap\SNCAScer\UAS.cFa or Hsap\SNCAScer\UAS.cKa under the control of Scer\GAL4elav-C155 can be restored by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP.

The increased mean size of synaptic vesicles as well as the shift in frequency distribution toward larger vesicles at both type 1b and 1s of neuromuscular junction boutons characteristic for third instar larvae expressing either Hsap\SNCAScer\UAS.cFa or Hsap\SNCAScer\UAS.cKa under the control of Scer\GAL4elav-C155 can be suppressed by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP.

The defective crawling behavior (decrease in distance travelled per unit of time) characteristic for third instar larvae expressing Hsap\SNCAScer\UAS.cFa under the control of either Scer\GAL4C164 or Scer\GAL4ple.PF can be suppressed by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP using the Scer\GAL4C164 driver and partially suppressed when the Scer\GAL4ple.PF driver is used.

The defective crawling behavior (decrease in distance travelled per unit of time) characteristic for third instar larvae expressing Hsap\SNCAScer\UAS.cKa under the control of either Scer\GAL4C164 or Scer\GAL4ple.PF can be suppressed by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP using either of the two drivers.

The loss of dopaminergic PPL1 neurons in the adult brain characteristic for flies expressing Hsap\SNCAScer\UAS.cFa under the control of either Scer\GAL4elav-C155 or Scer\GAL4ple.PF can be reversed by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP using either of the two drivers.

The loss of dopaminergic PPL1 neurons in the adult brain characteristic for flies expressing Hsap\SNCAScer\UAS.cKa under the control of Scer\GAL4elav-C155 can be reversed by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP.

The decrease in the number of rhabdomeres in the adult eye observed in flies expressing Hsap\SNCAScer\UAS.cFa under the control Scer\GAL4GMR.PU can be partially restored by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP.

The defects in climbing behavior seen in adult flies or various ages (10, 20 and 30 day-old) expressing either Hsap\SNCAScer\UAS.cFa or Hsap\SNCAScer\UAS.cKa under the control of Scer\GAL4elav-C155 can be suppressed by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP.

The defects in climbing behavior seen in adult flies or various ages (10, 20 and 30 day-old) expressing Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4ple.PF are suppressed by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP in 30 day-old flies only.

The shortened lifespan characteristic for adult flies expressing either Hsap\SNCAScer\UAS.cFa or Hsap\SNCAScer\UAS.cKa under the control of Scer\GAL4elav-C155 can be reversed by co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP.

Xenogenetic Interactions
Statement
Reference

Co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP almost completely rescues the wings defects caused by expression of Vcho\ctxAScer\UAS.cGa under the control of Scer\GAL4Bx-MS1096.

Co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP suppresses the emaciated, reduced body weight phenotype seen in flies expressing Vcho\ctxAScer\UAS.cGa under the control of Scer\GAL4Myo31DF-NP0001 in the presence of Scer\GAL80ts.αTub84B at 22[o]C (a permissive temperature for Scer\GAL80ts.αTub84B that reduces but does not eliminate Scer\GAL4Myo31DF-NP0001 activity).

Co-expression of Rab11Scer\UAS.T:Avic\GFP-EGFP reduces the size of the gaps seen between midgut intestinal epithelial cells in flies expressing Vcho\ctxAScer\UAS.cGa under the control of Scer\GAL4Myo31DF-NP0001 during the adult stage (temperature sensitive Scer\GAL80ts.αTub84B allele used to limit expression).

Expression of Rab11Scer\UAS.T:Avic\GFP-EGFP suppresses the reduction in average synaptic vesicle diameter seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

Expression of Rab11Scer\UAS.T:Avic\GFP-EGFP suppresses the reduction in eEJP and mEJP amplitudes seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155 to levels comparable with controls. No significant difference is seen in mEJP decay kinetics, mEJP frequency, mean resting membrane potential or quantal content compared to controls.

Expression of Rab11Scer\UAS.T:Avic\GFP-EGFP suppresses the third instar larval crawling defects seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4C164. The number of turns and distance travelled is comparable to controls.

Overexpression of Rab11Scer\UAS.T:Avic\GFP-EGFP almost completely suppresses the Hsap\HTTQ93.ex1p.Scer\UAS eclosion phenotype, with a greater than twofold increase in emergence.

Overexpression of Rab11Scer\UAS.T:Avic\GFP-EGFP gives a highly significant 43% suppression of the rhadomere degeneration seen upon expression of Hsap\HTTQ93.ex1p.Scer\UAS under the control of Scer\GAL4elav-C155.

Overexpression of Rab11Scer\UAS.T:Avic\GFP-EGFP increases median survival of Hsap\HTTQ93.ex1p.Scer\UAS by 50% from 10 to 15 days.

Expression of Rab11Scer\UAS.T:Avic\GFP-EGFP in the wing disc primordium suppresses the activity of Zzzz\EFScer\UAS.cGa (when both are under the control of Scer\GAL4Bx-MS1096).

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Rab11Scer\UAS.T:Avic\GFP-EGFP
Rab11UAS.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (27)