FlyBase Allele Report: Dmel\BubR1[K1204A]

General Information

Symbol

Dmel\BubR1^K1204A

Species

D. melanogaster

Name

FlyBase ID

FBal0362055

Feature type

allele

Associated gene

Dmel\BubR1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Tang et al., 2020)

Progenitor genotype

Cytology

Description

Amino acid substitution (K1204A) in the C-terminal kinase domain. This change has previously been shown to obstruct binding of ATP and results in a kinase-dead protein.s

(Tang et al., 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

MNV

2R:5,970,233..5,970,235 [-]

Nucleotide change:

AAG5970235GCA

Reported nucleotide change:

AAG?GCA

Amino acid change:

K1204A | BubR1-PA

Reported amino acid change:

K1204A

Inserted_sequence:

GCA

(Tang et al., 2020)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage

(Tang et al., 2020)

decreased cell number | oogenesis

(Tang et al., 2020)

long lived

(Tang et al., 2020)

Phenotype Manifest In

adult enteroendocrine cell | decreased number

(Tang et al., 2020)

germarium cap cell | decreased number

(Tang et al., 2020)

intestinal stem cell | adult stage | decreased number

(Tang et al., 2020)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

BubR1^K1204A has decreased cell number | oogenesis phenotype, suppressible by Scer\GAL4^Tub.PU/InR^K414P.UAS

(Tang et al., 2020)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

BubR1^K1204A has intestinal stem cell | adult stage | decreased number phenotype, non-enhanceable by Scer\GAL4^Tub.PU/InR^K414P.UAS

(Tang et al., 2020)

Suppressed by

Statement

Reference

BubR1^K1204A has germarium cap cell | decreased number phenotype, suppressible by Scer\GAL4^Tub.PU/InR^K414P.UAS

(Tang et al., 2020)

BubR1^K1204A has adult enteroendocrine cell | decreased number phenotype, suppressible by Scer\GAL4^Tub.PU/InR^K414P.UAS

(Tang et al., 2020)

NOT suppressed by

Statement

Reference

BubR1^K1204A has intestinal stem cell | adult stage | decreased number phenotype, non-suppressible by Scer\GAL4^Tub.PU/InR^K414P.UAS

(Tang et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

BubR1^K1204A

(Tang et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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