FlyBase Allele Report: Uuuu\Ub[K0.UAS.6.Stop.Tag:HA]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA}

General Information

Symbol

Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA}

Species

u. unknown

Name

FlyBase ID

FBal0362081

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{UAS-HA-Ub6-Stop.K0}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Blount et al., 2020)

Progenitor genotype

Carried in construct

P{UAS-HA-Ub6-Stop.K0}

Cytology

Description

UAS regulatory sequences (from pWalium10.moe) drive expression of an unanchored, uncleavable chain of six tandem copies of Ubiquitin; the chain lacks the internal "GG" motifs that are necessary for isopeptide bond formation and dismantling by deubiquitinases. The poly-Ubiquitin chain also lacks a terminal "GG", meaning that the chain cannot itself be conjugated onto other proteins. All Lys residues within the chain have been mutated to the similar, but non-ubiquitinatable, amino acid Arg to prevent ubiquitination. The coding sequence is tagged at the N-terminal end with Tag:polyHis.

(Blount et al., 2020)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Tagged with

purification tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage, with Scer\GAL4^sqh.PW

(Blount et al., 2020)

partially lethal, with Scer\GAL4^Mef2.PR

(Blount et al., 2020)

partially lethal - majority die, with Scer\GAL4^elav-C155

(Blount et al., 2020)

partially lethal - majority die, with Scer\GAL4^repo.PU

(Blount et al., 2020)

short lived, with Scer\GAL4^elav-C155

(Blount et al., 2020)

short lived, with Scer\GAL4^Mef2.PR

(Blount et al., 2020)

short lived, with Scer\GAL4^repo.PU

(Blount et al., 2020)

some die during embryonic stage, with Scer\GAL4^sqh.PW

(Blount et al., 2020)

some die during larval stage, with Scer\GAL4^elav-C155

(Blount et al., 2020)

some die during larval stage, with Scer\GAL4^Mef2.PR

(Blount et al., 2020)

some die during larval stage, with Scer\GAL4^sqh.PW

(Blount et al., 2020)

some die during P-stage, with Scer\GAL4^elav-C155

(Blount et al., 2020)

some die during P-stage, with Scer\GAL4^Mef2.PR

(Blount et al., 2020)

some die during P-stage, with Scer\GAL4^repo.PU

(Blount et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^repo.PU, Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA} has short lived phenotype, suppressible | partially by Dif^HM05257, Scer\GAL4^repo.PU

(Blount et al., 2020)

Scer\GAL4^repo.PU, Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA} has short lived phenotype, suppressible | partially by Rel^HMS00070, Scer\GAL4^repo.PU

(Blount et al., 2020)

Scer\GAL4^repo.PU, Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA} has short lived phenotype, suppressible | partially by Scer\GAL4^repo.PU/cact^HM04020

(Blount et al., 2020)

Scer\GAL4^repo.PU, Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA} has short lived phenotype, suppressible | partially by dl^JF02825/Scer\GAL4^repo.PU

(Blount et al., 2020)

Scer\GAL4^repo.PU, Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA} has short lived phenotype, suppressible | partially by IKKβ^GL00058, Scer\GAL4^repo.PU

(Blount et al., 2020)

Other

Statement

Reference

Scer\GAL4^repo.PU, Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA}, key^GL00088 has lethal phenotype

(Blount et al., 2020)

Scer\GAL4^repo.PU, Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA}, key^HMC04952 has lethal phenotype

(Blount et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Uuuu\Ub^{K0.UAS.6.Stop.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections