FlyBase Allele Report: Zzzz\scFv[9.UAS.Tag:MYC,Tag:polyHis]

General Information

Symbol

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}

Species

a. artificial

Name

FlyBase ID

FBal0363905

Feature type

allele

Associated gene

Zzzz\scFv

Associated Insertion(s)

Carried in Construct

P{UAS-scFv9.myc.his}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Fernandez-Funez et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-scFv9.myc.his}

Cytology

Description

UAS regulatory sequences drive expression of the scFv9 single-chain variable fragment antibody; this recognizes and binds the N-terminal 16 amino acids of the Aβ peptide and binds both the Aβ40 and Aβ42 forms (PMID:17108166). The coding sequence is tagged with Tag:MYC and Tag:polyHis.

(Fernandez-Funez et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

single-chain variable fragment antibody

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^2xAPP.SP.UAS

(Fernandez-Funez et al., 2015)

modeled by Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR

(Fernandez-Funez et al., 2015)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^2xAPP.SP.UAS

(Martin-Peña et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Mus\scFv^{9.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} under the control of Scer\GAL4^ey-OK107 does not engender any mushroom body abnormalities.

(Fernandez-Funez et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^ey-OK107, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} is a suppressor | partially of abnormal locomotor behavior | progressive phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^elav.PU

(Fernandez-Funez et al., 2015)

Scer\GAL4^ey-OK107/Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} is a suppressor | partially of increased cell death | progressive phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^elav.PU

(Fernandez-Funez et al., 2015)

Scer\GAL4^ey-OK107, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} is a suppressor | partially of increased cell death | progressive phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^elav.PU

(Fernandez-Funez et al., 2015)

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU is a suppressor | partially of visible phenotype of Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}

(Fernandez-Funez et al., 2015)

Scer\GAL4^ey-OK107/Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} is a suppressor | partially of abnormal locomotor behavior | progressive phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^elav.PU

(Fernandez-Funez et al., 2015)

NOT Suppressor of

Statement

Reference

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^ey-OK107 is a non-suppressor of abnormal neuroanatomy phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2015)

Other

Statement

Reference

Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^ey-OK107, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} has abnormal neuroanatomy phenotype

(Fernandez-Funez et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PU, Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} has ommatidium phenotype, suppressible | partially by Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PU, Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} has lens phenotype, suppressible | partially by Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^ey-OK107, Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} has calyx of adult mushroom body phenotype, suppressible | partially by Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2015)

Suppressor of

Statement

Reference

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU is a suppressor | partially of ommatidium phenotype of Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU is a suppressor | partially of lens phenotype of Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU is a suppressor of ommatidium phenotype of Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}

(Fernandez-Funez et al., 2015)

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU is a suppressor of lens phenotype of Hsap\APP^{770.K670N.M671L.UAS.VTR}, Hsap\BACE1^UAS.VTR, Scer\GAL4^GMR.PU, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}

(Fernandez-Funez et al., 2015)

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU is a suppressor | partially of ommatidium phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^GMR.PU is a suppressor | partially of lens phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^GMR.PU

(Fernandez-Funez et al., 2015)

Scer\GAL4^ey-OK107/Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} is a suppressor | partially of Kenyon cell | progressive phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^elav.PU

(Fernandez-Funez et al., 2015)

Scer\GAL4^ey-OK107, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} is a suppressor | partially of Kenyon cell | progressive phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^elav.PU

(Fernandez-Funez et al., 2015)

NOT Suppressor of

Statement

Reference

Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis}, Scer\GAL4^ey-OK107 is a non-suppressor of mushroom body phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2015)

Other

Statement

Reference

Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^ey-OK107, Zzzz\scFv^{42.2.UAS.Tag:MYC,Tag:polyHis}, Zzzz\scFv^{9.UAS.Tag:MYC,Tag:polyHis} has mushroom body phenotype

(Fernandez-Funez et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\APP^{2xAPP.SP.Scer\UAS} with Mus\scFv^{9.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} partially suppresses the Hsap\APP^{2xAPP.SP.Scer\UAS} phenotype, with larger eyes and improved pigmentation. The eyes of these flies are better organized, with fewer fused ommatidia, and better differentiation of lenses with fewer broken lenses.

Compared with the co-expression of Hsap\APP^{770.K670N.M671L.Scer\UAS.VTR} and Hsap\BACE1^Scer\UAS.VTR, flies co-expressing Hsap\APP^{770.K670N.M671L.Scer\UAS.VTR}, Hsap\BACE1^Scer\UAS.VTR, and Mus\scFv^{9.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} using Scer\GAL4^GMR.PU exhibit larger and better-organized eyes with fewer fused ommatidia and no broken lenses.

Co-expression of the combination of Mus\scFv^{9.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} and Mus\scFv^{9.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} suppresses the size defects and the disorganization of eyes resulting from the co-expression of Hsap\APP^{770.K670N.M671L.Scer\UAS.VTR} and Hsap\BACE1^Scer\UAS.VTR using Scer\GAL4^GMR.PU.

Co-expression of the combination of Mus\scFv^{42.2.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} and Mus\scFv^{9.Scer\UAS.T:Hsap\MYC,T:Zzzz\His6} with Scer\GAL4^ey-OK107>Hsap\APP^{2xAPP.SP.Scer\UAS} produces dorsal and medial mushroom body lobes that are slightly larger than wild-type.

(Fernandez-Funez et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer