2385bp of robo1 upstream flanking sequence drives expression of a deleted form of robo1 that lacks both the second immunoglobulin-like domain and the third fibronectin repeat. 2192bp of robo1 downstream flanking sequence is also present. The protein is tagged directly upstream of the Ig1 domain with 4 copies of Tag:HA.
Scer\GAL4elav.PLu, robo1ΔIg2ΔFn3.Tag:HA, robo11, robo2UAS.Tag:HA,Tag:SS(wg) has axon | embryonic stage phenotype
Scer\GAL4elav.PLu, commUAS.cUa, robo1ΔIg2ΔFn3.Tag:HA has axon | embryonic stage phenotype
robo1ΔIg2ΔFn3.Tag:HA partially rescues robo11