FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ssp3M1
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General Information
Symbol
Dmel\ssp3M1
Species
D. melanogaster
Name
FlyBase ID
FBal0367098
Feature type
allele
Associated gene
Dmel\ssp3
Associated Insertion(s)
TI{TI}ssp3M1
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Wormser et al., 2021)
Mutagen
CRISPR/Cas9
(Wormser et al., 2021)
gene targeting by homologous recombination
(Wormser et al., 2021)
Progenitor genotype
Associated Insertion(s)
TI{TI}ssp3M1
Cytology
Description

Part of endogenous ssp3 is replaced with an attP site followed by a loxP-flanked Disc\RFP3xP3.cUa marker.

(Wormser et al., 2021)
Allele components
Component
Use(s)
Also carries
attP
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2L:18,940,336..18,942,846 [-]
Comment:

Estimated location of a mutation in which part of the ssp3 coding sequence is replaced with an attP site followed by a loxP-flanked Disc\RFP3xP3.cUa marker. The extent of the deletion was mapped as the region between the sgRNA sequences.

(Abdu, 2021.8.19)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Bardet-Biedl syndrome
      CEA
      (Wormser et al., 2021)
      model of  azoospermia
      CEA
      (Wormser et al., 2021)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Very few (if any) ssp3M1 onion stage spermatids are normal; most nuclei and nebenkerne are of very different sizes and the nucleus-nebenkern association is often disrupted. Virtually all meiotic divisions are abnormal: microtubules emanating from the centrosomes do not appear to interact properly with the chromosomes, so that the chromosomes are dispersed and sometimes found outside the spindle; several meiosis II spindles are 'empty' while other meiosis II spindles appear to contain more than four chromosomes; spermatids are unable to form regular central spindles.

      (Wormser et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ssp3KO
      (Wormser et al., 2021)
      ssp3M1
      (Wormser et al., 2021)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)