FB2026_02 , released June 18, 2026
Allele: Dmel\dsxM.3xFLAG
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General Information
Symbol
Dmel\dsxM.3xFLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0369396
Feature type
allele
Associated gene
Dmel\dsx
Associated Insertion(s)
TI{2A-EGFP}dsxM.3xFLAG
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Oliver and Bhaskar, 2021.9.3)
gene targeting by homologous recombination
(Oliver and Bhaskar, 2021.9.3)
Progenitor genotype
Associated Insertion(s)
TI{2A-EGFP}dsxM.3xFLAG
Cytology
Description

A 3xFLAG-T2A-EGFP-NLS tag cassette followed by a male stop TAG has been inserted at 3R:7930232..7930233 (release 6 coordinates), tagging the male isoform of the endogenous dsx locus. The 3xFLAG tag is expected to be incorporated into the male dsx isoform, while the T2A sequence promotes the separate translation of the EGFP-Tag:NLS(Unk) reporter.

(Oliver and Bhaskar, 2021.9.3)
Allele components
Component
Use(s)
Inserted element
TI{2A-EGFP}
Encoded product / tool
EGFP
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
insertion
3R:7,930,232..7,930,233 [-]
Comment:

A 3xFLAG-T2A-EGFP-NLS tag cassette followed by a male stop TAG has been inserted at 3R:7930232..7930233 (release 6 coordinates)

(Oliver and Bhaskar, 2021.9.3)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      dsxM.3xFLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)