FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rab35CRISPR
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General Information
Symbol
Dmel\Rab35CRISPR
Species
D. melanogaster
Name
FlyBase ID
FBal0370497
Feature type
allele
Associated gene
Dmel\Rab35
Associated Insertion(s)
TI{TI}Rab35CRISPR
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kohrs et al., 2021)
Mutagen
CRISPR/Cas9
(Kohrs et al., 2021)
gene targeting by homologous recombination
(Kohrs et al., 2021)
Progenitor genotype
Associated Insertion(s)
TI{TI}Rab35CRISPR
Cytology
Description

Region within Rab35 (-24bp relative to ATG of Rab35-RC isoform to +20bp relative to the first bp of the Rab35 stop codon) is replaced with a loxP cassette containing a Disc\RFP3xP3.cUa marker.

(Kohrs et al., 2021)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
X:20,157,565..20,158,971 [-]
Comment:

A region within Rab35 (-24bp relative to ATG of Rab35-RC isoform to +20bp relative to the first bp of the Rab35 stop codon) is replaced with a loxP cassette containing a Disc\RFP3xP3.cUa marker. (Reported as an 816bp deletion but description corresponds to a 1407bp region).

(Kohrs et al., 2021)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Rab35CRISPR has dorsal tracheal branch primordium phenotype, enhanceable by Rab27Gal4-KO

      (Camelo et al., 2022)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rab35CRISPR
      Rab35null
      (Ochi et al., 2022)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)