FlyBase Allele Report: Dmel\Drosp[CR70269-KO-TG4.1]

General Information

Symbol

Dmel\Drosp^{CR70269-KO-TG4.1}

Species

D. melanogaster

Name

FlyBase ID

FBal0371264

Feature type

allele

Associated gene

Dmel\Drosp

Associated Insertion(s)

TI{CRIMIC.TG4.1}Drosp^{CR70269-KO-TG4.1}

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Gene Disruption Project members, 2018-)

gene targeting by homologous recombination

(Gene Disruption Project members, 2018-)

Progenitor genotype

Associated Insertion(s)

TI{CRIMIC.TG4.1}Drosp^{CR70269-KO-TG4.1}

Cytology

Description

A TI{CRIMIC.TG4.1} DNA cassette has been inserted into CG17739, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. In addition, the coding exons of CG17739 downstream of the inserted gene trap cassette have been deleted. The TI{CRIMIC.TG4.1} cassette was inserted via the CRISPR/Cas-9 hybrid technique, using two gRNAs that target CG17739 : one targeted to a coding intron (TATATACTTTAATCCTGTAGCGG) and the other to a non-coding exon in the 3' UTR (AACTATGTAATCATTACCAATGG).

(Gene Disruption Project members, 2018-)

Allele components

Component

Use(s)

Inserted element

TI{CRIMIC.TG4.1}

gene trap

RMCE target element

Encoded product / tool

GAL4

binary expression system - driver

Also carries

attP

integrase target site

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion_site

2R:12,308,751..12,310,294 [+]

(Rojo-Cortés et al., 2026, Kanca et al., 2022, Gene Disruption Project members, 2018-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Drosp^{CR70269-KO-TG4.1} has abnormal neuroanatomy | dominant phenotype, enhanceable by LpR1^{CR70219-TG4.0}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} has abnormal neuroanatomy | dominant phenotype, enhanceable by LpR2^{CR70220-TG4.0}

(Rojo-Cortés et al., 2026)

Suppressed by

Statement

Reference

Drosp^{CR70269-KO-TG4.1} has abnormal neuroanatomy | dominant phenotype, suppressible by Hsap\RELN^{UAS.cRa.Tag:HA}/Scer\GAL4^{Drosp-CR70269-KO-TG4.1}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} has decreased size phenotype, suppressible | partially by Hsap\RELN^{UAS.cRa.Tag:HA}/Scer\GAL4^{Drosp-CR70269-KO-TG4.1}

(Rojo-Cortés et al., 2026)

Enhancer of

Statement

Reference

Drosp^{CR70269-KO-TG4.1} is an enhancer of abnormal neuroanatomy | dominant phenotype of LpR1^{CR70219-TG4.0}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} is an enhancer of abnormal neuroanatomy | dominant phenotype of LpR2^{CR70220-TG4.0}

(Rojo-Cortés et al., 2026)

Phenotype Manifest In

Enhanced by

Statement

Reference

Drosp^{CR70269-KO-TG4.1} has mushroom body phenotype, enhanceable by LpR1^{CR70219-TG4.0}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} has mushroom body phenotype, enhanceable by LpR2^{CR70220-TG4.0}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} has axon phenotype, enhanceable by LpR2^{CR70220-TG4.0}

(Rojo-Cortés et al., 2026)

Suppressed by

Statement

Reference

Drosp^{CR70269-KO-TG4.1} has mushroom body phenotype, suppressible by Hsap\RELN^{UAS.cRa.Tag:HA}/Scer\GAL4^{Drosp-CR70269-KO-TG4.1}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} has mushroom body medial lobe phenotype, suppressible by Hsap\RELN^{UAS.cRa.Tag:HA}/Scer\GAL4^{Drosp-CR70269-KO-TG4.1}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} has central brain phenotype, suppressible | partially by Hsap\RELN^{UAS.cRa.Tag:HA}/Scer\GAL4^{Drosp-CR70269-KO-TG4.1}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} has axon phenotype, suppressible by Hsap\RELN^{UAS.cRa.Tag:HA}/Scer\GAL4^{Drosp-CR70269-KO-TG4.1}

(Rojo-Cortés et al., 2026)

Enhancer of

Statement

Reference

Drosp^{CR70269-KO-TG4.1} is an enhancer of mushroom body phenotype of LpR1^{CR70219-TG4.0}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} is an enhancer of mushroom body phenotype of LpR2^{CR70220-TG4.0}

(Rojo-Cortés et al., 2026)

Drosp^{CR70269-KO-TG4.1} is an enhancer of axon phenotype of LpR2^{CR70220-TG4.0}

(Rojo-Cortés et al., 2026)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Drosp^{CR70269-KO-TG4.1} is rescued by Scer\GAL4^{Drosp-CR70269-KO-TG4.1}/Drosp^UAS.Tag:HA

(Rojo-Cortés et al., 2026)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93823

y¹ w^*; TI{CRIMIC.TG4.1}Drosp^{CR70269-KO-TG4.1}/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG17739^{CR70269-KO-TG4.1}

CG17739^{CR70269-TG4.1}

Drosp^{CR70269-KO-TG4.1}

Drosp^CR70269

(Rojo-Cortés et al., 2026)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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