FB2026_02 , released June 18, 2026
Allele: Dmel\CubnB2
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General Information
Symbol
Dmel\CubnB2
Species
D. melanogaster
Name
FlyBase ID
FBal0371572
Feature type
allele
Associated gene
Dmel\Cubn
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Atienza-Manuel et al., 2021)
Mutagen
CRISPR/Cas9
(Atienza-Manuel et al., 2021)
Progenitor genotype
Cytology
Description

Deletion/insertion producing a frameshift followed by a premature stop codon, resulting in expression of a truncated Cubn protein lacking most of the C-terminal region of Cubn, including the EGF repeats (165 wild type amino acids, followed by 1 novel amino acid).

(Atienza-Manuel et al., 2021)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
X:9,570,517..9,570,524 [-]
Inserted_sequence:

AATGAC

Comment:

CCTGCGAG replaced with AATGAC (net 2bp deletion) starting in codon P162 of Cubn, creating a stop codon and leading to early translation termination after the addition of one novel amino acid. (Note a slight discrepency between the reported amino acid position and the FlyBase analysis position).

(Atienza-Manuel et al., 2021)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      CubnB2 homozygous larval nephrocytes exhibit a decrease in the number of slit diaphragms in the cortical region, and are not strictly confined to the entrance of labyrinthine channels but are also found at internal ectopic positions; they also show acentric nuclei.

      (Atienza-Manuel et al., 2021)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      CubnB2 has nephrocyte diaphragm | larval stage phenotype, non-enhanceable by Cubn2E3-1/Cubn2E3-1

      (Atienza-Manuel et al., 2021)

      CubnB2 has nephrocyte | larval stage phenotype, non-enhanceable by Cubn2E3-1/Cubn2E3-1

      (Atienza-Manuel et al., 2021)
      NOT suppressed by
      NOT Enhancer of
      Statement
      Reference

      CubnB2/CubnB2 is a non-enhancer of nephrocyte diaphragm | larval stage phenotype of Cubn2E3-1

      (Atienza-Manuel et al., 2021)

      CubnB2/CubnB2 is a non-enhancer of nephrocyte | larval stage phenotype of Cubn2E3-1

      (Atienza-Manuel et al., 2021)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CubnB2
      (Atienza-Manuel et al., 2021)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)